UPLC-MS/MS法同时测定白芍饮片中10种成分  

Simultaneous determination of ten components in Radix Paeoniae Alba pieces by UPLC- MS/MS

作　　者：刘杰[1] 许文[1,2] 李煌[1] 张平平[1] 向青[1,2] 徐伟[1] 褚克丹[1] 

机构地区：[1]福建中医药大学药学院,福州350122 [2]福建中医药大学生物医药研发中心,福州350122

出　　处：《药物分析杂志》2015年第4期635-643,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：福建省科技厅重点项目(2012Y0041);福建省教育厅项目(JA12176);校管课题(X2013014;X2013015;X2014132-学科);福建省"大学生创新创业训练计划"项目(2014)

摘　　要：目的：建立超高效液相色谱串联质谱法（UPLC-MS/MS法）同时测定白芍饮片中10种主要成分（没食子酸、原儿茶酸、芍药苷亚硫酸酯、氧化芍药苷、儿茶素、没食子酸甲酯、芍药内酯苷、芍药苷、五没食子酰葡萄糖、苯甲酰芍药苷）的含量。方法：采用UPLC-MS/MS法,负离子多反应监测（MRM）模式进行含量测定。色谱条件：采用Waters CORTECS C18（2.1 mm×100 mm,1.6μm）色谱柱,以乙腈（A）-0.1%甲酸水（B）为流动相,梯度洗脱,流速0.25 m L·min-1,柱温45℃。结果：在所设定的色谱条件下,5 min内定量分析白芍中10种主要成分在考察的浓度范围内呈良好的线性关系（r〉0.996 6）;回收率和RSD分别在97.3%~102.0%和3.1%~5.1%范围内。结论：本研究所建立的同时测定白芍中10种主要成分（没食子酸,原儿茶酸,芍药苷亚硫酸酯,氧化芍药苷,儿茶素,没食子酸甲酯,芍药内酯苷,芍药苷,五没食子酰葡萄糖,苯甲酰芍药苷）的UPLC-MS/MS定量分析方法简便、快捷、准确,为白芍饮片质量控制提供新的技术手段。Objective： To establish a rapid,accurate UPLC- MS / MS method for the determination of 10 major components（ gallic acid,potocatechuic acid,paeoniflorin sulfonate,oxypaeoniflorin,catechin,methyl gallate,albiflorin,paeoniflorin,pentagalloylglucose,benzoylpaeoniflorin） in Radix Paeoniae Alba pieces. Methods： The UPLC- MS /MS assay was performed on a Waters CORTECS C18（ 2. 1 mm × 100 mm,1. 6 μm） column with acetonitrile- water（ containing 0. 1% formic acid） as the mobile phase by gradient elution at a flow rate of 0. 25 m L·min- 1. The column temperature was set at 45 ℃. MS detection was performed with multiple reaction monitoring（ MRM） mode using negative electrospray ionization. Results： Under the optimized chromatographic conditions,good separation of 10 target components were obtained within 5 min. Satisfactory linearity was achieved within the investigated linear range and with fine determination coefficient（ r 0. 996 6）,the overall recoveries were ranged from 97. 3%- 102. 0% with the RSD ranging from 3. 1%- 5. 1%. Conclusion： It is the first report of simultaneous analysis of 10 major components（ gallic acid,potocatechuic acid,paeoniflorin sulfonate,oxypaeoniflorin,catechin,methyl gallate,albiflorin,paeoniflorin,pentagalloylglucose,benzoylpaeoniflorin） in Radix Paeoniae Alba by using UPLC- MS / MS method,which affords a highly sensitive,specific,speedy and efficient method for quality control of Radix Paeoniae Alba pieces.

关 键 词：白芍 饮片 没食子酸 原儿茶酸 芍药苷亚硫酸酯 氧化芍药苷 儿茶素 没食子酸甲酯 芍药内酯苷 芍药苷 五没食子酰葡萄糖 苯甲酰芍药苷 超高效液相色谱串联质谱 

分 类 号：R917[医药卫生—药物分析学]