大庆龙凤湿地底泥氨氧化细菌与氨氧化古菌多样性分析  

Diversity of Ammonia-oxidizing Bacteria and Ammonia-oxidizing Archaea in Sediment of Longfeng Wetland in Daqing

作　　者：马茹霞 晏磊[1] 王国兴[1] 王长虹[1] 贾军[1] 邓兵[1] 沈婷婷[2] 王伟东[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]上海岩土工程勘察设计研究院有限公司

出　　处：《黑龙江八一农垦大学学报》2015年第2期69-73,78,共6页journal of heilongjiang bayi agricultural university

基　　金：国家自然科学基金(31270536);黑龙江省高校科技创新团队项目(2012TD006)

摘　　要：为研究湿地底泥中氨氧化细菌(Ammonia-Oxidizing Bacteria,AOB)与氨氧化古菌(Ammonia-Oxidizing Archaea,AOA)的群落多样性,构建AOB与AOA的amo A基因克隆文库,同时利用限制性片段长度多态性(RFLP)技术将克隆文库阳性克隆子进行归类并测序。结果发现,从湿地底泥中均得到3个AOB与AOA的可操作分类单元(OTU)。其中,所有的AOB均属于亚硝化单胞菌属(Nitrosomonas),而AOA属于泉古菌门(Crenarcharota),与河口沉积物等环境中获得的序列具有较高同源性。To study the diversity of ammonia-oxidizing bacteria（ AOB） and ammonia-oxidizing archaea（ AOA） in sediment of Longfeng wetland in Daqing,amo A clone libraries of AOB and AOA were constructed. The libraries were analyzed by PCR-based restriction fragment length polymer- phism（ RFLP） technique. The results showed that 3 OTUs of AOB and AOA were obtainted from Longfeng wetland sediment,respectively. All sequences of AOB belonged to Nitrosomonas,and all sequences of AOA belonged to Crenarchaeota,and it had the high sequence homology with the sequences that were obtained from estuary sediments.

关 键 词：湿地底泥 氨氧化细菌 氨氧化古菌 多样性 

分 类 号：X172[环境科学与工程—环境科学]