点击化学用于生物分子标记的研究进展（英文）  

Recent Progress on Application of "Click" Chemistry in Labeling of Biomolecules

作　　者：吴杰[1,2] 喻艳华[1,2] 

机构地区：[1]江汉大学交叉学科研究院 [2]光电化学材料与器件省部共建教育部重点实验室,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2015年第2期138-145,共8页Journal of Jianghan University：Natural Science Edition

摘　　要：自1999年Barry Sharpless提出点击化学的概念以来,其得到极大的关注和发展。主要介绍了点击化学的定义和分类,其中Cu(I)催化下炔基与叠氮可形成稳定的1,2,3-3氮唑化合物,这类反应的应用最为广泛。它在标记生物大分子方面的应用主要从4个方面展开,包括标记多肽和蛋白,DNA和RNA,细胞中的酯质和病毒表面。Since the concept of ＂click＂ chemistry was introduced by Barry Sharpless in 1999,it hasgained considerable attention and developed quickly. In this review,the definition and classification of ＂click＂ reaction have been introduced. Among the different types of ＂click＂ reaction, the application ofCu（I）-catalyzed Huisgen 1,3-dipolar cycloaddition of azides and terminal alkynes（Cu AAC）to form1,2,3-triazoles is remarkable. Its applications in labeling of biomolecules were reviewed from four parts：labeling of peptides and proteins,DNA and RNA,lipid in living cells,and viral surfaces.

关 键 词：点击化学 三氮唑 多肽 蛋白质 DNA RNA 活细胞酯质 病毒表面 

分 类 号：O641[理学—物理化学]