藤黄酸对结直肠癌LOVO细胞增殖和血管生成素样蛋白4表达的影响  

Effect of gambogic acid on colorectal cancer LOVO cell proliferation and ANGPTL4 expression

作　　者：魏建昌[1] 曹杰[1] 杨平[1] 曾山崎[1] 王成兴[1] 邱旭彬 陈华翠[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院胃肠外科,广东广州510180

出　　处：《中国医药导报》2015年第11期4-6,10,共4页China Medical Herald

基　　金：国家自然科学资金资助项目(编号81272556);广东省科技计划项目(编号2010B060900016)

摘　　要：目的研究藤黄酸对结直肠癌LOVO细胞生长增殖和血管生成素样蛋白4(ANGPTL4)表达的影响,探讨藤黄酸可能的抗肿瘤机制。方法采用不同浓度的藤黄酸干预结直肠癌LOVO细胞,分别于12、24、36 h后应用CCK-8法检测细胞增殖活性,流式细胞仪检测细胞周期分布和细胞凋亡情况,Western blot检测ANGPTL4蛋白表达。结果藤黄酸对结直肠癌LOVO细胞的生长和增殖具有抑制作用,该抑制作用呈现浓度依赖性和时间依赖性(P<0.05)。藤黄酸阻滞LOVO细胞于G2/M期,阻滞作用呈现浓度依赖性(P<0.05)。高浓度藤黄酸能够诱导LOVO细胞凋亡(P<0.05)。藤黄酸抑制LOVO细胞ANGPTL4蛋白表达,ANGPTL4蛋白表达随藤黄酸作用浓度的增加而减少(P<0.05)。结论藤黄酸能够抑制结直肠癌LOVO细胞的生长和增殖,阻滞LOVO细胞于G2/M期,并诱导细胞凋亡,其作用机制可能与抑制ANGPTL4表达相关。Objective To investigate the effect of gambogic acid on colorectal cancer LOVO cells proliferation and the expression of ANGPTL4, discuss the antitumor mechanism of gambogic acid. Methods LOVO cells were cultured with different concentrations of gambogic acid for 12, 24 and 36 h in vitro. CCK-8 was used to observe the change in the proliferation of LOVO cells. Flow cytometry（FCM） was applied to analyze cell-cycle kinetics and apoptosis of LOVO cells cultured with different concentrations of gambogic acid for 24 h. Western Blot was applied to detect the protein expression of ANGPTL4. Results Gambogic acid suppressed the proliferation of LOVO cells in a concentration- dependent and time-dependent manner（P〈0.05）. Gambogic acid arrested LOVO cells at G2/M stages in a concentration-dependent manner（P〈0.05）. Gambogic acid with high concerntration could induce apoptosis of LOVO cells（P〈0.05）.Gambogic acid suppressed the expression of ANGPTL4 protein, the level of ANGPTL4 protein decreased as concentration increased（P〈0.05）. Conclusion Gambogic acid can inhibit the proliferation of LOVO cells, arrest LOVO cells at G2/M stages and induce apoptosis. The mechanism is related to the suppression of ANGPTL4.

关 键 词：藤黄酸 结直肠癌 ANGPTL4 

分 类 号：R735.35[医药卫生—肿瘤]