响应面法优化提取益母草多糖工艺  

Optimization Ultrasound-assisted Extraction Technology of Polysaccharide from Baphicacanthis Cusiae by Response Surface Methodology

作　　者：陈庆奇[1] 郑少燕[1,2] 张丹雁[2] 

机构地区：[1]南方医科大学附属佛山市妇幼保健院药剂科,广东佛山528000 [2]广州中医药大学中药学院,广东广州510006

出　　处：《安徽农业科学》2015年第12期35-38,共4页Journal of Anhui Agricultural Sciences

基　　金：广东省科技厅产学研结合项目(2010B090400529);佛山市卫生和计生局医学科研课题(408239275162)

摘　　要：[目的]优化益母草多糖的最佳提取工艺条件。[方法]以多糖提取率为评价指标,选定超声提取温度、提取时间及固液比作为主要考察因素进行单因素试验,再通过3因素3水平Box-Behnken设计试验,建立益母草多糖提取率的二次多项回归方程,计算得到优化条件。[结果]益母草多糖优化提取条件为提取温度35.5℃、提取时间20.5 min、固液比1∶24(m/v,g/ml),益母草多糖的提取率为(7.10±0.13)%。[结论]此方法具有快捷、高效等优点,为益母草多糖的综合开发应用奠定理论基础。[ Objective ] To optimize extraction technology of polysaccharides from Leonurus japonicus Houtt （LJP） by response surface methodology, provide a basis for the further development of Leonurus polysaccharides. [ Method ] With the extraction yield of polysaccharide as the index of evaluation, selected the ultrasonic extraction temperature, time and solid - liquid ratio as the main factors. On the basis of single factor experiment, 3 factors 3 levels Box - Behnken Design was established. Established quadratic polynomial regression equation of extraction yield of LJP, calculated optimized conditions. [ Result ] The optimum extraction conditions of Leonurus polysaccharides were extraction time with 20. 5 min, the ratio of water to raw material was 24：1 （ v： m, ml/g） and temperature was 35.5 ℃. The observed maximal yield of LJP was （7. 10 ± 0.13 ）%. [ Conclusion] This method has the advantages of fast, high efficiency, the study will lay the theoretical foundation for the comprehensive development and application of LJP.

关 键 词：益母草 多糖 响应面分析法 超声辅助提取 

分 类 号：S567[农业科学—中草药栽培]