Efficient in Vitro Micropropagation System for Radish （Raphanus sativus L.） Var. Beeralu Rabu  

作　　者：Indeewari Manawadu Nilanthi Dahanayake Senanayakage Gamini Nonis Senanayake 

机构地区：[1]Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya 81110, Sri Lanka

出　　处：《Journal of Agricultural Science and Technology(B)》2014年第10期787-792,共6页农业科学与技术（B）

摘　　要：This study was conducted to investigate the effects of explants and hormone combinations on the callogenesis, calli growth and regeneration of local variety of radish （Beeralu Rabu）. As well as three explant types： hypocotyl, leaf and root, seven hormone combination based on Murashige and Skoog （MS） medium as 0.1, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/L 2,4-dichlorophenoxyacetic acid （2,4-D） with 0.1 mg/L 1-naphthaleneacetic acid （NAA） and without hormones （control） were employed. Then immerged calli were transferred to regenerate in MS with 0.1 mg/L NAA and 1.0, 1.5, 2.0, 2.5, 3.0 mg/L benzyl adenine （BA）. Healthy regenerated shoots were introduced to MS medium with 0.1, 0.25, 0.5, 0.75 and 1.0 mg/L indole-3-butyric acid （IBA） for rooting. After one month the callogenesis, diameter of fresh callus, shoots regeneration, number of regenerated shoots and roots were evaluated. Observations were made that, 0.5 mg/L 2,4-D with 0.1 mg/L NAA and hypocotyl explant of radish was the best showing significantly highest callus diameter （〉 2 cm）. Medium with 2.5 mg/L BA and 0.1 mg/L NAA was the best hormonal combination for the in vitro shoot regeneration （6.6 shoots/explant） while the highest number of roots were observed from MS medium with 0.25 mg/L.

关 键 词：Raphanus sativus CALLUS REGENERATION MS medium. 

分 类 号：S631.1[农业科学—蔬菜学] S828.3[农业科学—园艺学]