Biodiversity of Indigenous Amylolytic and Cellulolytic Bacteria in Sago Waste Product at Susupu, North Moluccas  

作　　者：Utami Sri Hastuti Pramitha Yakub Henny Nurul Khasanah 

机构地区：[1]Biology Department, FMIPA, State University of Malang, Malang 65145, Indonesia [2]Educational Biology, PPS, State University of Malang, Malang 65145, Indonesia

出　　处：《Journal of Life Sciences》2014年第11期920-924,共5页生命科学（英文版）

摘　　要：People at Susupu, North Moluccas prepare the sago （Metroxylon sago） in traditionally way for consumption. The residue of processed sago usually thrown away on the ground, so it was caused pollution. Some amylolytic bacteria species and cellulolytic bacteria species could be founded in sago waste product. The purpose of this research are：1 ） to identify the indigenous amylolytic bacteria species in sago waste product; 2） to identify the indigenous cellulolytic bacteria species in sago waste product; 3） to test the amylum hydrolysis ability of each amylolytic bacteria species; 4） to test the cellulose hydrolysis ability of each cellulolytic bacteria species. This research was conduct at the Microbiology laboratory, Biology Department-FMIPA-State University of Malang and the Microbiology laboratory-Faculty of Medicine-Brawijaya University. The research samples are sago waste product from Susupu, North Moluccas. The samples were grinded and diluted in nutrient broth, and then the suspension was diluted gradually until 10^-10. The suspension was inoculated 0.1 mL each on nutrient agar medium in 37℃ during 1 × 24 h. Each bacteria colony that grows on the medium were isolated and determined to know which one were the amyloliytic bacteria and the cellulolytic bacteria. The amylum hydrolysis index of each amylolytic bacteria species were counted as well as the cellulose hydrolysis index of each cellulolytic bacteria species. Each amylolytic bacteria and cellulolytic bacteria species were identified. This research result shows that： 1） there are 5 indigenous amylolytic bacteria species, i.e., Bacillus mycoides, Bacillus cereus, Bacillus licheniformis, Bacillus alvei and Serratia liquefaciens; 2） there are 4 indigenous cellulolytic bacteria species, i.e., Serratia liquefaciens, Acinetobacter iwofii, Bacillus licheniformis and Bacillus cereus; 3） Serratia liquefaciens has the highest amylum hydrolysis index, i.e., 3.08; 4） Acinetobacter iwoffii has the highest cellulose hydrolysis index, i.e., 2.01

关 键 词：Amylolytic bacteria cellulolytic bacteria sago waste product. 

分 类 号：X131[环境科学与工程—环境科学] TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]