PEG预处理对盐胁迫下水稻幼苗抗氧化酶活性的影响  

Effect of PEG Pretreatment on Antioxidant Enzymes Activity under Salt Stress in Rice Seedlings

作　　者：张诗婉 魏玮[1] 梅映学 卜宁[1] 李雪梅[1] 马纯艳[1] 马莲菊[1] 

机构地区：[1]沈阳师范大学化学与生命科学学院,辽宁沈阳110034

出　　处：《环境科学与技术》2015年第5期7-10,共4页Environmental Science & Technology

基　　金：国家自然科学基金项目(31070285);北方粳稻遗传育种省部共建教育部重点实验室/农业部东北水稻生物学与遗传育种重点实验室(沈阳农业大学)开放课题;沈阳师范大学生态与环境研究中心主任基金(EERC-K-201406)

摘　　要：以辽星1号水稻幼苗为试验材料,采用10%PEG-6000模拟干旱预处理3 d,复水3 d后,再用100 mmol/L Na Cl进行盐胁迫处理3 d,测定水稻幼苗叶片丙二醛含量、和抗氧化酶SOD、POD、CAT和APX活性等。结果表明,PEG预处理后的水稻幼苗,再对其进行盐胁迫处理后,其MDA的含量为1.30(U/(mol·g Fw)),盐胁迫处理组的MDA含量为1.57(U/(mol·g Fw)),由数据看出PEG预处理组与盐胁迫组相比MDA的含量显著降低(P<0.05),说明PEG预处理可以减小水稻幼苗叶片的伤害。由图可以看出,其抗氧化酶SOD、POD、CAT和APX活性均显著高于盐胁迫处理组(P<0.05),PEG预处理可以增强水稻叶片清除活性氧的能力,从而提高水稻幼苗的耐盐性。Liaoxing 1 rice seedlings were used as experimental material. Rice seedlings were first treated for3 d with 10% PEG-6000, then rewatered for 3 d, and added 100 mrnol/L NaC1 to medium for 3 d, for the purpose to determine leaf water content, MDA content, antioxidant enzyme SOD, POD, CAT and APX activity. Results showed that under the rice seedling after PEG pretreatment and salt stress treatment, MDA content was 1.30 （U/tool ·g Fw）, and salt stress treatment＇s MDA content was 1.57 （U/mol.g Fw）, it showed that MDA content by PEG pretreatment was significantly reduced compared with that by salt stress （P〈0.05）, which indicated that PEG pretreatment had less damage to the leaves of rice seedlings, and its relative water content, antioxidant enzyme SOD, CAT, POD and APX activities in leaves were higher than those by salt stress （P〈0.05）. PEG nretreatment could enhance the salt tolerance of rice seedlinzs.

关 键 词：PEG预处理 水稻 保护酶 盐胁迫 

分 类 号：X173[环境科学与工程—环境科学]