甜菜属厚皮菜SWTY-1细胞质VNTRs多态性分析  

Analysis for variable number of tandem repeats in cytoplasm genome of Beta vulgaris cicla SWTY-1

作　　者：刘巧红[1] 罗成飞[1] 代翠红[1] 袁肖寒[2] 梁乃国[1] 刘天骄[1] 史淑芝[1] 于歆[1] 吕星道 

机构地区：[1]哈尔滨工业大学食品科学与工程学院,哈尔滨150090 [2]东北农业大学生命科学与生物技术研究中心,哈尔滨150030

出　　处：《生物信息学》2015年第1期9-12,共4页Chinese Journal of Bioinformatics

基　　金：国家自然科学基金(No.31371685)

摘　　要：利用数目可变串联重复序列(Variable Number of Tandem Repeats,VNTRs)微卫星标记方法,对重庆厚皮菜甜菜材料SWTY-1群体中100个单株的细胞质线粒体DNA片段中TR2位点VNTRs片段多态性进行分析。结果显示97个单株线粒体TR2位点微卫星串联重复序列均为3拷贝,与普通糖甜菜一致;3个单株线粒体TR2位点微卫星串联重复序列为6拷贝,发现甜菜属厚皮菜细胞质TR2位点VNTRs存在多态性,在该群体中发现了不同于甜菜栽培种新的细胞质单株。对该群体材料100个单株的抽薹及结籽进行观测,结果显示微卫星串联重复序列为6拷贝的变异植株中2个单株花期未抽苔开花,1株抽苔晚未形成正常种子;细胞质TR2位点VNTRs片段拷贝数为3的植株中2个单株未能正常抽薹,其他植株均正常抽薹结籽。Molecular identification for cytoplasm polymorphism of Beta vulgaris cicla L materials SWTY-1 was conducted in this paper. Polymorphisms in mt DNA TR2 locus of Beta vulgaris cicla SWTY-1 were analyzed using variable number of tandem repeats（VNTRs） molecular markers method. The result showed that the numbers of tandem repeats in mt DNA TR2 locus were three-copy array of tandem repeats in 97 single plants among the 100 plants of Swiss chard,which was consistent with sugar beet. Six-copy VNTRs in mt DNA TR2 locus were found in 3single plants among the population. Two plants could not bolt among the three plants whose VNTRs copy numbers were six. One plant bolting late could not form normal seeds among the three plants whose VNTRs copy number were six. Only two plant among the 97 plants with three-copy in TR2 locus failed to bolt normally and others bolt normally and form seeds.

关 键 词：甜菜属 细胞质 雄不育 线粒体 分子标记 

分 类 号：TS242.2[轻工技术与工程—制糖工程]