依托咪酯对急性脑缺血大鼠海马CA1区细胞凋亡与胱天蛋白酶-3及胱天蛋白酶-10蛋白表达的影响  

Effects of etomidate on acute cerebral ischemia in rat hippocampal CA1 cells apoptosis and the caspase-3 and caspase-10 protein expression influence

作　　者：蒲国华[1] 李宝永[1] 刘铁军[1] 

机构地区：[1]河北联合大学附属医院麻醉科,唐山063000

出　　处：《山西医药杂志》2015年第6期631-633,共3页Shanxi Medical Journal

摘　　要：目的探讨依托咪酯对急性脑缺血大鼠海马CA1区细胞凋亡与胱天蛋白酶（caspase）-3及胱天蛋白酶（caspase）-10表达的影响。方法线栓法制作大鼠大脑中动脉缺血（MCAO）再灌注模型。应用原位末端标记法检测细胞凋亡,蛋白印迹法检测caspase-3及caspase-10蛋白表达。结果 1脑缺血组大鼠海马CA1区3h即出现凋亡阳性细胞,48h达到高峰,72h凋亡阳性细胞的数量开始下降。依托咪酯组大鼠海马CA1区凋亡细胞数于给药后再灌注6～72h各时间点均低于脑缺血组（P〈0.05）。2依托咪酯组大鼠海马CAl区caspase-3及caspase-10蛋白表达于给药后再灌注6--72h各时间点均低于脑缺血组（P〈0.05）。结论依托咪酯能够抑制急性脑缺血损伤引起的细胞凋亡。Objective To investigate the effect of etomidate on acute cerebral ischemia in rat hippocampal CA1 cells apoptosis and the caspase-3and caspase-10 expression influence.Methods With intraluminal suture method in rats with middle cerebral artery ischemia reperfusion model（MCAO）.Application of in situend labeling method to detect apoptosis,Western-blotting assay for detection of caspase-3and caspase-10 protein expression.Results 1Cerebral ischemia rats hippocampal area CA1 is of 3happearance of apoptosis positive cells,48 h peak,72 hapoptosis positive cells began to decline.Etomidate group hippocampal CA1 area of rats after administration of the number of apoptotic cells in reperfusion 6-72 hat each time point were lower than those in ischemia group（P〈0.05）.2The etomidate group in hippocampal CA1 region of rats with caspase-3and caspase-10 protein expression in after the administration of 6-72 hreperfusion time at each time point were lower than those in ischemia group（P0.05）.Conclusion Etomidate can inhibit acute cerebral ischemia induced cell apoptosis.

关 键 词：脑缺血 依托咪酯 细胞凋亡 半胱氨酸天冬氨酸蛋白酶 

分 类 号：R743.31[医药卫生—神经病学与精神病学]