神经肽Y对小神经胶质细胞活化状态和生成TNF-α的影响  

Effect of NPY on the biological activity of microglia and production of TNF-α in these cells

作　　者：李琦军[1] 吴永波[2] 常军英[1] 侯卫星[1] 邢兆国[1] 王彦志[1] 穆卫庐[1] 李炎[1] 贾东昭[1] 张淑丽 

机构地区：[1]河北省石家庄市第三医院,河北石家庄050011 [2]河北省石家庄市公安局法医损伤检验鉴定室,河北石家庄050011 [3]河北省秦皇岛市骨科医院,河北秦皇岛066001

出　　处：《现代中西医结合杂志》2015年第13期1384-1387,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的探讨神经肽Y(NPY)对原代小神经胶质细胞生物活性及生成肿瘤坏死因子-α(TNF-α)的影响。方法培养原代大鼠皮质小胶质细胞,经脂多糖(LPS)和NPY处理后行免疫细胞化学荧光染色,显微镜下观察LPS和NPY对小胶质细胞形态学的影响。将小胶质细胞分为对照组、LPS组、NPY+LPS组、NPY组和BIBP3226+NPY+LPS组,每组3个样本,培养各组细胞6 h。ELISA法检测培养液中TNF-α蛋白含量,RT-PCR方法检测小胶质细胞中TNF-αmRNA表达水平。结果 LPS处理后小胶质细胞处于活化状态,NPY处理后的小胶质细胞活化水平降低。LPS组和IBP 3226+NPY+LPS组培养液中TNF-α蛋白含量及细胞中TNF-αmRNA表达水平显著高于对照组(P均<0.05);LPS+NPY组TNF-α蛋白含量和TNF-αmRNA表达水平明显低于LPS组和IBP3226+NPY+LPS组(P均<0.05)。结论 NPY能够降低小神经胶质细胞的生物活性。NPY可能通过激活NPY Y1受体抑制小神经胶质细胞生成TNF-α。Objective It is to explore the effect of NPY on biological activity of primary microglia and the production of TNF-α in the rats. Methods Primary cerebral cortical microglia were cultured and treated with LPS and NPY. Then they were stained by immunocytochemistry staining and the morphological characteristics of microglia was observed with microscope. The microglia were divided into control group, LPS group, NPY ＋ LPS group, NPY group and BIBP3226 ＋ NPY ＋ LPS group. Every group had 3 samples and the the ceils were cultured for 6 h. The protein levels of TNF - α in the culture media and the mRNA expression levels of TNF -α in the microglia cells of different groups were detected with the methods of ELISA and RT - PCR respectively. Results The microglia treated with LPS kept active, the activity of microglias treated with NPY was lower. The contents of TNF - α in the culture media and the mRNA expression levels of TNF - α in the ceils of LPS group increased remarkably compared with control group（ P 〈 0.05 ）. Compared with LPS group, the contents of TNF -α in the culture media and the mRNA expression levels of TNF - α in the cells of LPS ＋ NPY group reduced obviously. Compared with LPS ＋ NPY group, the contents of TNF - α in the culture media and the mRNA expression levels of TNF -α in the cells of BIBP3226 ＋ NPY ＋ LPS group rose outstandingly （ P 〈 0.05 ）. Conclusion NPY can restrain the biological activity of microglia cells. NPY can reduce the production of TNF-α of microglia cells, Activation of NPY Y1 recepter on the microglia cells may be one of the reasons.

关 键 词：神经肽-Y 小神经胶质细胞 肿瘤坏死因子-Α 

分 类 号：R-332[医药卫生]