睾酮联合阿那曲唑对三阴乳腺癌细胞MDA-MB-231的抑制作用的研究  

The inhibitory effects of testosterone combined with anastrozole on breast cancer cells MDA- MB- 231

作　　者：陈榕[1] 崔军威[2] 韦伟[2] 

机构地区：[1]北京大学深圳医院药剂科,广东深圳518036 [2]北京大学深圳医院乳腺外科,广东深圳518036

出　　处：《吉林医学》2015年第10期1996-1999,共4页Jilin Medical Journal

基　　金：深圳市科技计划项目资助课题[课题编号:201103010]

摘　　要：目的:评估睾酮(Testosterone)联合芳香化酶抑制剂阿那曲唑及单用睾酮对雄激素受体(Androgen receptor,AR)阳性的乳腺癌细胞MDA-MB-231(ER-/AR+)作用的效果。方法:配置不同浓度的药物,CCK-8检测药物作用不同时间后细胞增殖抑制率,流式细胞凋亡分析试剂盒检测药物对细胞凋亡影响,荧光定量PCR法检测不同浓度药物作用下MDA-MB-231细胞中hsa-miRNA-30e表达情况。结果:体外实验中阿那曲唑单药及低浓度睾酮对MDA-MB-231细胞起到促增殖作用,高浓度睾酮及联合用药组会抑制MDA-MB-231细胞增殖作用,并且联合用药组较睾酮单药组增殖抑制率(P<0.05)及细胞凋亡率高(P<0.01);分析72h后不同药物浓度下hsa-miRNA-30e表达情况后显示,睾酮及联合用药组较阿那曲唑单药组可以明显降低其表达水平(P<0.01)。结论:睾酮联合芳香化酶抑制剂对AR阳性乳腺癌细胞具有较强的抑制作用,并且其作用机制可能通过miRNA调节途径发挥作用。Objective To evaluate the inhibitory effects of testosterone combined with anastrozole on breast cancer cells MDA - MB -231 （ER - / AR ＋ ）. Method The different concentrations of drugs containing testosterone,anastrozole and combined drugs had been prepared with dimethyl sulfoxide. Perform CCK -8 and flowed cytometric detection with Annexin V/ PI double dyeing methods to examine the prolifera-tion and apoptosis of cells respectively. The expression of hsa - miRNA -30e under the different concentrations of drugs in 72 h had been de-tected through real - time PCR. Results In vivo experiment testosterone with low concentration and anastrozole promoted proliferation of MDA- MB -231 cells,but testosterone with high concentration and combination drugs could suppress the cells proliferation in 72 h. Testosterone and combination drugs had also significantly reduced the expression levels of hsa - miRNA - 30e than the anastrozole group in 72 h（P ﹤0. 01）. Conclusion Testosterone combined with anastrozole have stronger inhibitory effect on AR positive breast cancer cells,and the mecha-nism of drugs action may be connected with regulation of miRNA.

关 键 词：乳腺癌 睾酮 芳香化酶抑制剂 hsa-miRNA-30e 

分 类 号：R737.9[医药卫生—肿瘤]