结合HPLC-3D图谱的不同产地延胡索生物碱指纹图谱分析  

HPLC Fingerprinting Coupled with HPLC-3D Chromatogram Analysis of Alkaloids in Corydalis yanhusuo from Different Regions

作　　者：徐皓[1] 王哲[1] 赵桦[1] 昝丽霞[1] 蒋景龙[1] 

机构地区：[1]陕西理工学院生物科学与工程学院,汉中723000

出　　处：《天然产物研究与开发》2015年第4期567-571,共5页Natural Product Research and Development

基　　金：陕西省教育厅重点实验室科研计划(13JS020);陕西理工学院校级科研项目(slgky13-40);陕西省重点学科专项建设资金(2014)

摘　　要：采用HPLC指纹图谱技术并结合HPLC-3D图谱分析13个不同产地延胡索药材中生物碱成分,并以5种主要生物碱作为指标,采用相似度评价和非共有峰的特征进行聚类分析。结果表明:通过对延胡索HPLC-3D图谱和二维指纹图谱的特征分析,13个产地延胡索的特征共有峰有12个,图谱相似度均在90%以上。非共有峰的系统聚类分析显示不同来源延胡索的三个聚类群,其中安徽-亳州、广西-河池和辽宁-清原被分为一类;湖北-郧西、山东-泰安和黑龙江-绥化被分为一类;河南-辉县、湖南-新化、吉林-通化、浙江-东阳、浙江-磐安、浙江-仙居和汉中-城固被分为一类。HPLC二维指纹图谱结合HPLC-3D图谱能够更全面的反映药材中的成分构成,在质量控制中有重要意义。In this study, HPLC fingerprinting coupled with HPLC-3D chromatogram analysis was carried out to investigate the alkaloid components in Corydalis yanhusuo from 13 different regions. Cluster analysis was performed based on similarity and uncommon peaks gotten from 5 alkaloids. As the resuh, 12 characteristic peaks were detected in samples from 13 regions,the similarity of their fingerprinting was higher than 0.9. At the same rime,cluster analysis based on uncommon peaks showed that the samples can be clustered into three groups. Among them, samples from Bozhou Anhui, Guangxi Hechi and Liaoning Qingyuan were clustered one group, samples from Yunxi Hubei, Shandong Tonghua, and Heilongjian Suihua joined together in one group, while the last group contained the samples from Zhejiang Dongyang, Zhejiang Panan, Zhejiang Xianju and Hanzhong. The study indicated the components of C,. yanhuso can be reflected by HPLC fingerprinting coupled with HPLC-3D ehromatogram analysis,which was of great use in the quality control of this Chinese traditional medicine.

关 键 词：延胡索 生物碱 HPLC指纹图谱 聚类分析 

分 类 号：Q503[生物学—生物化学]