紫菜多糖体外抗紫外线辐射活性研究  

Study on the Anti-ultraviolet Activity of Porphyra Polysaccharides in vitro

作　　者：叶翠芳[1] 王巧利[1] 刘秋英[1] 利奕成[1] 

机构地区：[1]暨南大学生物医药研究开发基地,广州510632

出　　处：《天然产物研究与开发》2015年第4期641-644,666,共5页Natural Product Research and Development

基　　金：广东省海洋经济创新发展区域示范专项(GD2012-D01-002);广东省海洋渔业科技推广专项(A201301C06)

摘　　要：主要研究紫菜多糖体外抗紫外线辐射活性,探讨紫菜多糖在紫外辐射方面的应用前景。用UVA辐射损伤小鼠成纤维细胞NIH 3T3,用MTT法检测紫菜多糖对小鼠成纤维细胞的作用,用流式细胞仪分析细胞周期分布。实验结果表明,紫菜多糖≤100μg/m L时,对小鼠成纤维细胞无明显的毒性,有显著的促增殖效果,并且能提高受UVA辐射损伤的小鼠成纤维细胞的存活率;紫菜多糖可使细胞周期的G1期比例减少,G2期的比例增加。由此可见,紫菜多糖对体外培养的因紫外辐射损伤的小鼠成纤维细胞有明显的修复作用。The objective of this study was to investigate the effects of Porphyra polysaccharides on mouse fibroblast cell line NIH 3T3 exposed to ultraviolet radiation and to discuss the application of Porphyra Polysaccharides in cosmetic protecting against ultraviolet radiation. NIH 3T3 was cultured to build the model of cell lesion caused by UVA ray in vitro. MTT assay was carried out to study cell proliferation and cell cycle was analyzed by flow cytometry. As a result, Porphyra polysaccharides have no obvious cytotoxlcity on NIH 3T3 when its concentration was 100 μg/mL or less,but with good proliferation effect;In addition,Porphyra polysaccharides can significantly decrease the Gt phase ratios and increase G2 phase ratios. Thus ,it was concluded that Porphyra polysaccharides have good repairing effect on NIH 3T3 exposing to ul- traviolet radiation.

关 键 词：紫菜多糖 紫外线 小鼠成纤维细胞 

分 类 号：Q331[生物学—遗传学]