响应面法优选双水相萃取分离珠子草中木脂素hypophyllanthin的方法  

Optimization of Aqueous Two-phase Extraction of Lignans Hypophyllanthin from Phyllanthus niruri Using Response Surface Methodology

作　　者：曹洵[1] 刘盛[1] 周敏[1] 林小霞[1] 韦万兴[1] 

机构地区：[1]广西大学化学化工学院,南宁530004

出　　处：《天然产物研究与开发》2015年第4期720-725,共6页Natural Product Research and Development

基　　金：国家自然科学基金(81060261);广西自然科学基金(2012GXNSFAA053021)

摘　　要：通过乙醇-硫酸铵双水相体系,以盐浓度(w/w)、醇浓度(w/w)、超声提取时间(min)为响应因素,根据中心组合(Box-Benhnken)试验设计原理,采用三因素三水平响应面分析法确定木脂素hypophyllanthin的最佳分配体系,并在此基础上用环己烷对上相进行液液选择性萃取纯化hypophyllanthin。结果表明,双水相体系提取木脂素hypophyllanthin的最佳条件为:盐浓度26.54%、醇浓度21%、超声提取时间为20 min。在此条件下,hypophyllanthin的分配系数可达75.82,对上相再次萃取hypophyllanthin的产率达3.18 mg/g,高于传统柱层析色谱法。此方法操作简单、绿色环保、成本低廉,为木脂素hypophyllanthin的分离提供了新的方法。In this study, an aqueous two-phase system （ATPS） based on ethanol/（NH4 ）2 SO4 was evaluated for the extraction of lignans from Phyllanthus niruri. Box-Behnken design （BBD） was used to study the effects of salt concentration, ethanol concentration and ultrasonic extraction time on the partition behavior of main bioactive components lignans hypophyllanthin. The results showed 26.54% （w/w） （NH4） 2SO4,21% （w/w） ethanol,20 rain of ultrasonic extraction for hypophyllanthin were the optimal conditions with partition coefficient of 76.62. Furthermore, the cyclohexane was used for another step extraction on upper phase,under which 3.18 mg/g hypophyllanthin were obtained. The ATPS was firstly used to separate and purify hypophyllanthin from P. niruri and higher yields were obtained comparing with traditional column chromatography. The developed ATPS method was easier,greener and cheaper. It provided a new approach for the separation of hypophyllanthin.

关 键 词：双水相 中心组合 木脂素 hypophyllanthin 

分 类 号：O629.9[理学—有机化学]