RNAi介导ObR基因沉默对人乳头状甲状腺癌K1细胞增殖与侵袭能力的影响  

Effect on the proliferation and invasion of human papillary thyroid carcinoma K1 cells after RNAi mediated knockdown

作　　者：王帅[1,2] 孔霞[3] 张国安[4] 侯森[5] 崔文[5] 

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250062 [2]山东省医学科学院,济南250022 [3]济宁医学院附属济宁市第一人民医院,山东济宁272011 [4]济宁医学院基础学院 [5]济宁医学院法医学与医学检验学院,济宁272067

出　　处：《济宁医学院学报》2015年第2期99-102,共4页Journal of Jining Medical University

基　　金：山东省自然科学基金(编号:2009ZRB02352);济宁市科技发展计划(编号:2011-31);济宁医学院青年基金(编号:JYQ 2011KM006);山东省高等学校计划项目(编号:J13LKI4);山东省高校优秀科技创新团队计划资助

摘　　要：目的探讨用RNAi介导瘦素受体基因(ObR)沉默人乳头状甲状腺癌K1细胞(K1细胞)增殖与侵袭能力的影响。方法使用siRNA干扰技术瞬时转染构建siRNA-ObR。瞬时转染后,Real-time PCR和Western blotting在mRNA水平和蛋白水平检测siRNA-ObR是否有效抑制K1细胞内靶基因的表达;MTT实验研究ObR沉默后K1细胞的增殖能力;Transwell实验检测ObR沉默后K1细胞的侵袭能力。结果 Realtime PCR实验结果显示,ObR基因沉默后K1细胞ObR mRNA表达显著降低;Western blotting实验结果显示,ObR基因沉默后K1细胞ObR蛋白表达显著降低;MTT实验结果显示ObR对K1细胞的增殖没有意义;Transwell实验结果显示ObR沉默后K1细胞侵袭能力显著下降。结论 ObR基因沉默能有效抑制K1细胞ObR基因的表达,降低K1细胞的侵袭能力。Objective To study the effect of RNAi-mediated OBR gene silencing on human papillary thyroid carcinoma cell K1.Methods siRNA interference technology transient transfection was used to construction of siRNA-ObR.After transfection,we detected whether siRNA-ObR inhibited the expression of target genes in mRNA level and protein level.MTT and Transwell respectively detected proliferation and invasion of K1 cells after OBR gene knockdown.Results Real-time PCR results showed that the level of mRNA expression decreased in K1 cells after ObR gene silencing.Western Blotting results exhibited that the level of protein expression was inhibited by ObR gene silencing.MTT results showed the silencing of ObR did not influence the proliferation of K1 cells.Transwell results demonstrated the silencing of ObR decreased K1 cells invasion.Conclusion OBR siRNA could effectively inhibit the expression of target gene and reduce invasive ability of human papillary thyroid carcinoma cell K1.

关 键 词：RNAI 基因沉默 SIRNA 人乳头状甲状腺癌K1细胞 OBR基因 

分 类 号：R736.1[医药卫生—肿瘤]