替芬泰对HepG2-2.2.15细胞HBV-DNA的抑制作用  

Inhibitory Effect of Tifentai on HBV-DNA in Cultured Cell Line Hep G2-2.2.15 in vitro

作　　者：王盼丽[1,2] 刘青川[2] 王晨吟[1] 黄正明[2] 

机构地区：[1]贵阳中医学院药学院,贵州贵阳550000 [2]解放军302医院药学部药理研究室,北京100039

出　　处：《解放军药学学报》2015年第1期1-3,9,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金："重大新药创新"科技重大专项"十二五"计划;No.2011ZX09102-009-02

摘　　要：目的探讨替芬泰对Hep G2-2.2.15细胞HBV-DNA的抑制作用。方法以Hep G2-2.2.15细胞作为实验模型,通过MTT法检测替芬泰对Hep G2-2.2.15细胞的毒性,确定替芬泰对Hep G2-2.2.15细胞的半数中毒浓度(TC50)。在替芬泰浓度为50μg·ml-1时未见明显的细胞毒性,所以设替芬泰3个剂量组为50、25、12.5μg·ml-1,拉米夫定50μg·ml-1作为阳性对照药,收集给药3、6 d的细胞。用实时荧光定量PCR法检测DNA载量。结果替芬泰对Hep G2-2.2.15细胞的TC50为180.70μg·ml-1。随着替芬泰药物浓度的增加,Hep G2-2.2.15细胞内的HBV-DNA拷贝数相应减少,并有明显的浓度依赖性。结论替芬泰对Hep G2-2.2.15细胞内HBV-DNA有显著的抑制作用,有效浓度为12.5μg·ml-1,最佳浓度为50μg·ml-1。Objective To investigate the inhibitory effect of Tifentai（ TFT） on HBV-DNA in cultured cell line Hep G2-2. 2. 15 in vitro. Methods The median toxic concentration（ TC50） of TFT was explored using Hep G2-2. 2. 15 cells toxicity experiment. The dosage of TFT was 50,25 and 12. 5 μg·ml^-1respectively,while lamivudine50 μg·ml^-1was used as positive control. Cells were collected at 3 d and 6 d. Real-time fluorescent quantitative PCR method was used to detect the loads of HBV-DNA. Results The TC50 of TFT was 180. 70 μg·ml^-1,in Hep G2-2. 2. 15 cells. The number of copies of HBV-DNA Hep G2-2. 2. 15 cells decreased with the increase of drug concentration of TFT in a dose-dependent manner. Conclusion TFT can inhibit HBV-DNA in Hep G2-2. 2. 15 cells,the effective concentration is 12. 5 μg·ml^-1while the optimal concentration is 50 μg·ml^-1.

关 键 词：替芬泰 乙型肝炎病毒 HEP G2-2.2.15细胞 HBV-DNA 

分 类 号：R965[医药卫生—药理学]