奈达铂联合BI-1基因沉默对鼻咽癌细胞增殖和凋亡的影响  

Nasopharyngeal carcinoma cells proliferation and apoptosis induced by Nedaplatin and silence BI-1 gene

作　　者：罗海清[1] 李祥勇[2] 蔡宏华[3] 刘付梅[2] 崔仲宜 官成浓[1] 

机构地区：[1]广东医学院附属医院肿瘤中心二区,湛江524001 [2]广东医学院生物化学与分子生物学教研室,湛江524023 [3]广东医学院附属医院心电图室,湛江524001

出　　处：《齐齐哈尔医学院学报》2015年第10期1416-1418,共3页Journal of Qiqihar Medical University

基　　金：广东省医学科研基金(B2012280)

摘　　要：目的研究奈达铂联合靶向沉默BI-1基因对鼻咽癌细胞CNE-2增殖和凋亡的影响。方法采用MTT法检测奈达铂联合靶向沉默BI-1基因对鼻咽癌细胞CNE-2增殖的抑制作用。采用流式细胞技术检测奈达铂联合靶向沉默BI-1基因对鼻咽癌细胞CNE-2凋亡的影响。结果奈达铂联合靶向沉默BI-1基因呈时间和剂量依赖性抑制鼻咽癌细胞株CNE-2的增殖,奈达铂联合靶向沉默BI-1基因呈剂量依赖性诱导鼻咽癌细胞CNE-2的凋亡。结论研究结果证实奈达铂联合靶向沉默BI-1基因能有效抑制鼻咽癌细胞CNE-2的增殖,并能诱导鼻咽癌细胞CNE-2的凋亡。这种化疗联合靶向治疗的模式以期为鼻咽癌的治疗提供一种新的高效低毒的治疗模式。Objective The effect of Nedaplatin and silence BI-1 gene on proliferation and apoptosis of CNE-2 Nasopharyngeal carcinoma cells was studied. Methods Nedaplatin combined with silence BI-1 gene inhibited proliferation of CNE-2 Nasopharyngeal carcinoma cells,as detected using tetrazolium colorimetric assay.Cellular apoptosis of CNE-2 Nasopharyngeal carcinoma cells induced by Nedaplatin combined with silence BI-1gene was measured by flow cytometry. Results Nedaplatin combined with silence BI-1 gene showed inhibition of proliferation of CNE-2 Nasopharyngeal carcinoma cells in a dose-and time-dependent manner. Moreover Nedaplatin combined with silence BI-1 gene could promote apoptosis of CNE-2 Nasopharyngeal carcinoma cells in a dose-dependent manner. Conclusions The chemotherapy combined with targeted therapy will provide a new treatment mode with high efficiency and low toxicity for treatment of nasopharyngeal carcinoma.

关 键 词：奈达铂 BI-1基因 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤]