对玉米螟具有生物活性的海洋放线菌筛选与鉴定  

Screening and Identification of Marine Actinomyces with Bioactive for Asian Corn Borer(Ostrinia furnacalis G.)

作　　者：刘志航[1] 袁忠林[1] 罗兰[1] 

机构地区：[1]青岛农业大学农学与植物保护学院/山东省植物病虫害综合防控重点实验室,山东青岛266109

出　　处：《玉米科学》2015年第2期147-151,共5页Journal of Maize Sciences

基　　金：国家级大学生创新创业训练计划(201210435002);"十二五"农村领域国家科技计划课题(BAD19B04-09);山东省高等学校青年骨干教师国内访问学者项目;山东省"泰山学者"建设工程专项;青岛农业大学第六届大学生创新教育立项经费

摘　　要：通过平板稀释法对来自青岛不同地区的海洋样品进行分离,共得到18株海洋放线菌。采用浸虫法测定海洋放线菌发酵液对玉米螟2龄幼虫的室内毒力,筛选得到2株活性较高的菌株YC3和SLR3,其48 h发酵液对玉米螟2龄幼虫的校正死亡率分别为86.21%和81.04%。根据形态特征、培养特征及生理生化性状将活性菌株鉴定为链霉菌属(Streptomyces)。Eighteen strain marine actinomyces were isolated from sea samples of Qingdao different regions byusing dilution-plate method. Toxicities of fermentation broth from these marine actinomyces were assayed against 2instar larvae of Asian corn borer by insect immersion bioassay method, and two strains had insecticidal activities for Ostrinia furnacalis G. The corrected mortality rates were 86.21% and 81.04% respectively in 48 h after treatmentwith supernatant from fermentation broth of YC3 and SLR3. The morphological and cultural characteristics, physio-logical and biochemical properties showed two strains were Streptomyces.

关 键 词：玉米 放线菌 发酵液 玉米螟 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]