口腔鳞状细胞癌和癌旁组织的基因表达分析  

Gene expression analysis of oral squamous cell carcinoma and adjacent tissues

作　　者：周颖 姜毅华[2] 

机构地区：[1]成都市成飞医院口腔科,成都610091 [2]中国科学技术大学生命科学学院系统生物学系,合肥230027

出　　处：《国际口腔医学杂志》2015年第3期310-313,共4页International Journal of Stomatology

摘　　要：目的利用来自美国国立生物信息技术中心(NCBI)基因表达数据库(GEO)的基因芯片数据,分析基因在口腔鳞状细胞癌(OSCC)中癌和癌旁组织的差异表达情况,为寻找癌和癌旁组织双边的抑癌靶标提供依据。方法选取来自NCBI-GEO数据库中OSCC患者的癌和癌旁组织,以及正常人口腔组织的基因芯片数据,分别计算得到前两者相对后者的差异表达基因,并进行功能聚类与特定基因的通路分析。结果大部分差异基因为癌与癌旁所共有,其中多数基因的差异表达方向在两者中一致,少部分方向不一致。如WIF1基因在癌组织中表达下调,但在癌旁组织中表达上调。这种差异表达行为的不同与基因的功能相关,因此,差异表达行为可能为抑癌靶标的发现提供线索。结论基因在癌和癌旁组织中的差异表达行为与基因的功能相关,具有特定差异表达行为的基因可能成为潜在的抑癌靶标。Objective This study aims to provide evidence for the discovery of double-sided cancer-inhibitory targets by analyzing the behavior of gene differential expression in oral squamous cell carcinoma（OSCC）＇s cancer and adjacent tissues. The tissues used in the study are deposited at the National Center for Biotechnology Information（NCBI）-gene expression omnibus（GEO）. Methods Expression data of OSCC cancer and adjacent tissues, as well as normal oral tissues, were downloaded from the NCBI-GEO database. After identifying differentially expressed genes, functional clustering and pathway analysis were performed. Results Most of the genes differentially expressed in cancer and adjacent tissues were similar in terms of normal oral tissues; most of these tissues have the same differential expression direction. The behavior of gene differential expression was related to its function. However, the WIF1 sample, which theoretically suppresses cell cycle, was actually up-regulated in cancer adjacent tissue. Conclusion The relationship between the genes＇ function and their differential expression directions could provide evidence for double-sided target searching.

关 键 词：口腔鳞状细胞癌 双边药物靶标 功能聚类 

分 类 号：R739.8[医药卫生—肿瘤]