铜离子还原能力法结合星点设计-效应面法优选锁阳抗氧化物质的提取工艺  

Optimal Extraction Process of Antioxidant Substances from Cynomorium songaricum by CUPRAC and Central Composite Design-Response Surface Methodology

作　　者：李辉[1,2] 王晓飞[2] 刘铭佩[2] 韩姗姗[1,2] 焦海胜[2] 

机构地区：[1]兰州大学药学院,兰州730030 [2]兰州大学第二医院,兰州730030

出　　处：《中国药房》2015年第13期1821-1824,共4页China Pharmacy

基　　金：中央高校基本科研业务费专项资金(No.lzujbky2013-46);兰州大学第二医院院内中医药项目(No.YJzy2013-30)

摘　　要：目的:优化锁阳中抗氧化物质的提取工艺。方法:以液料比、乙醇体积分数、提取时间为自变量,对锁阳提取物采用铜离子还原能力(CUPRAC)法测得的抗氧化活性为因变量,用星点设计-效应面法预测最优提取工艺。结果:预测的最优提取工艺条件为液料比13∶1、乙醇体积分数50%、提取时间1.5 h。经验证,该工艺提取的锁阳提取物抗氧化活性没食子酸量为51.00 mg/g,与预测值49.68 mg/g比较偏差较小。结论:应用CUPRAC法结合星点设计-效应面法能够有效优选锁阳抗氧化物质的提取工艺。OBJECTIVE： To optimize the extraction process of antioxidant substances from Cynomorium songaricum. METH- ODS： With the independent variables of liquid-material ratio, the volume fraction of ethanol and extraction time, the dependent variable of antioxidant activity was determined by Cupric reducing antioxidant power （CUPRAC）. Central composite design-re- sponse surface methodology was conducted to predict the optimal extraction process. RESULTS： The predicted extraction process of C. songaricum was as follows as the liquid-material ratio of 13 ： 1, the volume fraction of ethanol of 50% and extraction time of 1.5 h. It was verified that the amount of anfioxidant activity of gallic acid from C. songaricum was 51.00 mg/g, which had small deviation when compared with the prediction value of 49.68 mg/g. CONCLUSIONS： The combination application of CUPRAC and central composite design-response surface methodology can effectively optimize the extraction process of antioxidant substances from C. songaricum and the optimized extraction process is applicable for the extraction of antioxidant substances from C. songaricum.

关 键 词：锁阳 星点设计-效应面法 CUPRAC法 抗氧化活性 提取工艺 优化 

分 类 号：R283[医药卫生—中药学] R284.2[医药卫生—中医学]