OK-432肿瘤细胞疫苗对DBA/2荷瘤小鼠CD4^+CD25^+调节性T细胞免疫效应研究  

Immune effects of OK-432 tumor vaccine on the CD4^+CD25^+ Treg cells of tumor mice in DAB/2 tumor-bearing mice in DBA/2 tumor-bearing mice

作　　者：武峰[1] 李琰[1] 田国兵[1] 

机构地区：[1]山西医科大学口腔医院修复科,太原030001

出　　处：《中国药物与临床》2015年第3期334-336,共3页Chinese Remedies & Clinics

基　　金：国家自然科学基金(81141091)

摘　　要：目的探讨OK-432肿瘤细胞疫苗对DBA/2小鼠CD4+CD25+Treg细胞免疫效应。方法本研究利用7,12-二甲基苯蒽(DMBA)/巴豆油二阶段诱癌法建立DBA/2小鼠皮肤癌荷瘤模型,将荷瘤小鼠随机分为3组,分别为OK-432疫苗组,OK-432组和空白对照组,各组小鼠于左侧腹腔分别注射浓度为5×105个/100μl的OK-432肿瘤细胞疫苗100μl,0.7 KE/100μl的OK-432 100μl磷酸盐缓冲液(PBS)液100μl,每周注射1次,连续注射3周,比较和分析不同实验组皮肤癌荷瘤小鼠的免疫应答。结果药物末次干预后第1、3、7、14天,OK-432疫苗组和空白对照组相比CD4+CD25+Treg细胞明显降低,差异均具有统计学意义(P<0.05)。结论本实验证实OK-432肿瘤疫苗能减少CD4+CD25+Treg细胞的含量,可以降低机体免疫抑制功能。Objective To investigate the immune effects of OK-432 tumor cell vaccine on the CD4＋CD25＋Treg cells in DBA/2 mice. Methods The 7, 12-DMBA/croton oil two-stage method of carcinogenesis was used to build DBA/2 mice tumor-bearing model of skin cancer. And the tumor-bearing mice were randomly divided into three groups, which were the OK-432 vaccine group, OK-432 group and control group. The mice in each group were injected with 100 μl OK-432 tumor cell vaccine（5×10^5/100 μl concentration）, OK-432（0.7 KE/100 μl） and phosphate buffer saline（PBS） respectively on the left abdominal cavity once a week for continuous three weeks. The immune re-sponses of skin cancer tumor-bearing mice in different experimental groups were compared and analyzed. Results At days 1, 3, 7, 14 after the last drug intervention, the CD4＋CD25＋Treg cells were significantly reduced in the OK-432 vaccine group than that in the control group, with statistically significant differences（ P 〈0.05）. Conclusion Our experiment confirmed that the OK-432 tumor vaccine could reduce the content of CD4＋CD25＋Treg cells and body immune suppression function.

关 键 词：癌症疫苗 小鼠 近交DBA T淋巴细胞 调节性 流式细胞术 

分 类 号：R73-36[医药卫生—肿瘤]