耐低温降解生物质废弃物复合菌剂的混料设计优化  

Optimal mixture design of preparation of cold-adapted multiple species inoculant for biomass waste degradation

作　　者：罗立津[1,2,3] 万立 陈宏[1] 徐福乐 贾纬[1] 聂毅磊[1] 温翠莲[4] 

机构地区：[1]福建省微生物研究所,福建省新药(微生物)筛选重点实验室,福州350007 [2]中国农业大学农业生物技术国家重点实验室,中国农业大学生物学院,北京100193 [3]福建超大现代农业科技研究所,福州350003 [4]福州大学材料科学与工程学院,福州350108

出　　处：《科技导报》2015年第8期24-29,共6页Science & Technology Review

基　　金：福州市科技计划项目(2013-G-97);福州大学校科技发展基金项目(2012-xy-1)

摘　　要：为了筛选制备耐低温高效降解农业生物质废弃物的复合菌剂,在已获得的耐低温木质纤维素降解菌群A25-3的基础上,采用混料设计(mixture design)对复合菌比例进行优化,通过测定菌群A25-3、酿酒酵母(Saccharomyces cerevisiae)和绿色木霉(Trichoderma viride)不同比例搭配对纤维素酶(CMCase、滤纸酶和外切葡聚糖酶)、蛋白酶和α-淀粉酶活性的影响,建立了各菌株(群)搭配比例与试验指标之间的回归方程。使用Design Expert 8.0软件的优化功能对满足所有期望的响应值进行优化,得到复合菌的最优配比(质量分数)为绿色木霉37.24%,菌群A25-3 67.76%。对该最优比例进行验证试验,得到的结果与预测值基本一致。秸秆降解试验表明,固体复合菌剂可高效降解秸秆,有效提高堆肥体系的温度,在低温环境下的堆肥和秸秆腐熟具有很好的应用前景。In this paper, an optimum mixture design is used for the preparation of cold-adapted multiple species inoculant. Based on the Design Expert software, a quadratic model is established as a function of the component fractions of species, such as the Saccharomyces cerevisiae, the Trichoderma viride and the flora A25-3, on the enzyme activity of the CMCase, the FPase, the cellobiohydrolase, the proteinase and the a＇amylase. The response values satisfying all expectations are optimized, and the most excellent combinations of the Saccharomyces cerevisiae, the Trichoderma viride and the flora A25-3 are 0%, 37.24% and 67.76%, respectively. The result of the verification experiment on the formulation is consistent with the prediction. The straw degradation experiments show that the multiple species inoculant has a high level of degradation efficiency for the straw at a low temperature. It suggests that there is a great application prospect of the multiple species inoculant under cold conditions.

关 键 词：混料设计 复合菌剂 纤维素酶 耐低温 生物质废弃物 

分 类 号：X712[环境科学与工程—环境工程]