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作 者:刘小蔓[1,2] 常增荣[2] 戴俊东[1] 陈有根[2] 傅欣彤[2] 郭洪祝[2]
机构地区:[1]北京中医药大学中药学院,北京100102 [2]北京市药品检验所,北京100035
出 处:《中国新药杂志》2015年第8期954-957,共4页Chinese Journal of New Drugs
基 金:北京市科委课题(Z111100056811045)
摘 要:目的:建立牡丹皮中没食子酸、芍药苷、五没食子酰葡萄糖和丹皮酚4种化学成分的HPLC含量测定方法。方法:采用Cosmosil 5C18-MS-II色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.2%甲酸为流动相进行梯度洗脱,流速1.0 m L·min^-1,芍药苷检测波长为230 nm,其余3种成分检测波长为274 nm,柱温30℃。结果:没食子酸、芍药苷、五没食子酰葡萄糖和丹皮酚进样量与峰面积分别在0.106 6~1.066μg(r=0.999 7),0.138 0~1.380μg(r=1.000 0),0.105 6~1.056μg(r=1.000 0),0.542 4~5.424μg(r=0.999 5)线性良好;平均回收率(n=6)分别为103.2%,97.0%,100.7%,97.8%。结论:该方法准确可靠、重复性好,可为牡丹皮的质量控制与评价提供参考。Objective: To establish an HPLC method for the content determination ot gallic acla, pacoolflorin, 1 ,2,3,4,6-pentagalloyglucose and paeonol in root bark of Paeonia suffruticosa. Methods: The determina- tion was performed on a Cosmosil 5C 18-MS-II column (250 mm× 4.6 mm ,5 μm) ; the mobile phase was acetonitrile (A)-0.2% formic acid solution (B) with gradient elution at a flow rate of 1.0 mL· min^-1. The detection wave- length was 274nm (gallic acid, 1,2,3,4,6-pentagalloyglucose, paeonol) and 230 nm (paeoniflorin)- The colume temperature was 27 ℃. Results : The 4 compounds in sample solution were stable. Good linearity (r = 0. 999 5 1. 000) was observed in the standard curves of gallic acid, paeoniflorin, 1,2,3,4,6-pentagalloyglucose and paeonol in the ranges of 0. 106 6 - 1.066 μg, 0. 1380 - 1. 380μg, 0. 105 6 - 1.056μg and 0.5424-5.424 μg, respectively. The average recoveries (n = 6) were 103.2% , 97.0% , 100.7% , and 97.8% , respectively. Conclu sion : This method is dependable, simple and practical. It can be used to provide a reference for the quality control of Paeonia suffruticosa.
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