The Stripe Rust Resistance Gene Yr10 Encodes an Evolutionary-Conserved and Unique CC-NBS-LRR Sequence in Wheat  

作　　者：Wei Liu Michele Frick Rene Huel Cory L. Nykiforuk Xiaomin Wang Denis A. Gaudet Francois Eudes Robert L. Conner Alan Kuzyk Zhensheng Kang Andre Laroche 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling,Shaanxi 712100, RR. China [2]Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st Avenue South, Lethbridge, Alberta T1J 4B1, Canada [3]Current address: The International Commission on Missing Persons, Alipasina 45A, Sarajevo 71000, Bosnia and Herzegovina [4]Current address: Emergent BioSolutions, 155 Innovation Drive, Winnipeg, MB R3T 5Y3, Canada [5]Current address: Agriculture and Agri-Food Canada, Morden Research Centre, Unit 100-101, Route 100, Morden, MB R6M 1Y5, Canada [6]Retired from Agriculture and Agri-Food Canada

出　　处：《Molecular Plant》2014年第12期1740-1755,共16页分子植物（英文版）

摘　　要：The first seedling or all-stage resistance （R） R gene against stripe rust isolated from Moro wheat （Triticum aes- tivum L.） using a map-based cloning approach was identified as Yr10. Clone 4B of this gene encodes a highly evolutionary- conserved and unique CC-NBS-LRR sequence. Clone 4E, a homolog of Yr10, but lacking transcription start site （TSS） and putative TATA-box and CAAT-box, is likely a non-expressed pseudogene. Clones 4B and 4E are 84% identical and divergent in the intron and the LRR domain. Gene silencing and transgenesis were used in conjunction with inoculation with differen- tially avirulent and virulent stripe rust strains to demonstrate Yr10 functionality. The Yr10 CC-NBS-LRR sequence is unique among known CC-NBS-LRR R genes in wheat but highly conserved homologs （E = 0.0） were identified in Aegilops tauschii and other monocots including Hordeum vulgare and Brachypodium distachyon. Related sequences were also identified in genomic databases of maize, rice, and in sorghum. This is the first report of a CC-NBS-LRR resistance gene in plants with limited homologies in its native host, but with numerous homologous R genes in related monocots that are either host or non-hosts for stripe rust. These results represent a unique example of gene evolution and dispersion across species.The first seedling or all-stage resistance （R） R gene against stripe rust isolated from Moro wheat （Triticum aes- tivum L.） using a map-based cloning approach was identified as Yr10. Clone 4B of this gene encodes a highly evolutionary- conserved and unique CC-NBS-LRR sequence. Clone 4E, a homolog of Yr10, but lacking transcription start site （TSS） and putative TATA-box and CAAT-box, is likely a non-expressed pseudogene. Clones 4B and 4E are 84% identical and divergent in the intron and the LRR domain. Gene silencing and transgenesis were used in conjunction with inoculation with differen- tially avirulent and virulent stripe rust strains to demonstrate Yr10 functionality. The Yr10 CC-NBS-LRR sequence is unique among known CC-NBS-LRR R genes in wheat but highly conserved homologs （E = 0.0） were identified in Aegilops tauschii and other monocots including Hordeum vulgare and Brachypodium distachyon. Related sequences were also identified in genomic databases of maize, rice, and in sorghum. This is the first report of a CC-NBS-LRR resistance gene in plants with limited homologies in its native host, but with numerous homologous R genes in related monocots that are either host or non-hosts for stripe rust. These results represent a unique example of gene evolution and dispersion across species.

关 键 词：seedling or all-stage stripe rust resistance gene functionality TRANSGENESIS gene silencing homolog sequences coiled-coil region nucleot de-b nding site leucine-rich repeat domain. 

分 类 号：Q987[生物学—遗传学] S512.1[生物学—人类学]