TRANSGENESIS

作品数：25被引量：88H指数：5
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相关领域：农业科学生物学更多>>
相关作者：石德顺孟凡丽陆凤花王丹崔奎青更多>>
相关机构：广西壮族自治区人口和计划生育研究中心广西大学潍坊市人民医院更多>>
相关期刊：《American Journal of Plant Sciences》《Neural Regeneration Research》《Frontiers of Agricultural Science and Engineering》《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》更多>>
相关基金：国家自然科学基金国家重点基础研究发展计划国家教育部博士点基金国家高技术研究发展计划更多>>
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Establishment of highly efficient transgenic system for black soldier fly(Hermetia illucens)被引量：1
《Insect Science》2023年第4期888-900,共13页Zongqing Kou Xingyu Luo Yuguo Jiang Bihui Chen Yu Song Yaohui Wang Jun Xui Jeffery K.Tomberlin Yongping Huang 
supported by the National Natural Science Foundation of China(32021001,31830093);Strategic Priority Research Program of Chinese Academy of Sciences(Grant no.XDPB16).
The black soldier fly(BSF),Hermetia illucens,is a promising insect for miti-gating solid waste problems as its larvae are able to bioconvert organic waste into valuable biomass.We recently reported a high-quality geno...
关键词：actin5C promoter black soldier fly hyPBase TRANSGENESIS U6 promoter 
GoldenFish:a rapid and efficient system to customize constructs for zebrafish transgenesis
《Journal of Molecular Cell Biology》2022年第12期63-66,共4页Zhanmei Jiang Lirong Huang Jieqiong Zhao Yanfeng Li Jianlong Ma Rui Ni Qifen Yang Lingfei Luo Yun Yang Jingying Chen 
supported by the National Natural Science Foundation of China(32270859,32000576,and32192400);the National Key R&D Program of China(2021YFA0805000);the Natural ScienceeFoundationofChongqing(cstc2020jcyj-msxmX0882).
Transgenesis, which inserts exogenous DNA into animal genomes, is a widely used technique. Traditionally, the constructs for transgenesis are generated by step-by-step subcloning of DNA fragments, which requires multi...
关键词：FISH GOLDEN SYSTEM 
Biotechnological interventions for the sustainable management of a global pest,whitefly(Bemisia tabaci)被引量：1
《Insect Science》2021年第5期1228-1252,共25页Archna Suhag Honey Yadav Darshna Chaudhary S.Subramanian Ranjana Jaiwal Pawan K.Jaiwal 
Whiteflies(Bemisia tabaci)are polyphagous invasive hemipteran insects that cause serious losses of important crops by directly feeding on phloem sap and transmitting pathogenic viruses.These insects have emerged as a ...
关键词：Bemisia tabaci. gene editing management NANOTECHNOLOGY RNAI TRANSGENESIS 
Manipulation of spermatogonial stem cells in livestock species被引量：7
《Journal of Animal Science and Biotechnology》2019年第4期833-850,共18页Filipp Savvulidi Martin Ptacek Karina Savvulidi Vargova Ludek Stadnik 
supported by the S grant of the Ministry of Education,Youth and Sport(MEYS)of Czech Republic;supported by the Primus Research Programme PRIMUS/17/MED/16 of the Charles University
We are entering an exciting epoch in livestock biotechnology during which the fundamental approaches(such as transgenesis, spermatozoa cryopreservation and artificial insemination) will be enhanced based on the modern...
关键词：CRISPR/Cas9 Genome editing LIVESTOCK TRANSGENESIS Long-term culture systems Male GERMLINE STEM cells Recipient preparation Sertoli CELLS Spermatogonial STEM CELLS Ultrasonographic-guided CANNULATION 
Transient expression of the enhanced green fluorescent protein(egfp) gene in Sargassum horneri被引量：2
《Journal of Oceanology and Limnology》2019年第2期651-656,共6页PANG Yunlong LI Yan LIU Zhengyi CUI Yulin QIN Song 
Supported by the National Key Research and Development Program of China(No.2016YFC1402106);the Project of Innovation&Development of Marine Economy(HHCL201803);the National Natural Science Foundation of China(Nos.41406192,41376139);the Science and Technology Service Network Initiative of Chinese Academy of Sciences(No.KFJ-STS-ZDTP-023)
Sargassum horneri is a macroalga widespread in North Asia-Pacific region, and these years its bloom has caused huge damage to the environment and the economic in China. To make up the blank on genetic engineering rese...
关键词：green FLUORESCENT protein (GFP) particle BOMBARDMENT SARGASSUM horneri TRANSGENESIS 
Local transgene expression and whole-body transgenesis to model brain diseases in nonhuman primate被引量：2
《Animal Models and Experimental Medicine》2019年第1期9-17,共9页Lucie Chansel-Debordeaux Erwan Bezard 
Animal model is an essential tool in the life sciences research, notably in understanding the pathogenesis of the diseases and for further therapeutic intervention success. Rodents have been the most frequently used a...
关键词：ANIMAL models NEUROSCIENCE nonhuman PRIMATES 
Development of genome engineering technologies in cattle: from random to specific被引量：6
《Journal of Animal Science and Biotechnology》2018年第2期285-293,共9页Soo-Young Yum Ki-Young Youn Woo-Jae Choi Goo Jang 
National Research Foundation of Korea(NRF-2017R1A2B3004972);IPET(No.109023–05-5-CG000);The BK21 PLUS Program for Creative Veterinary Science Research
The production of transgenic farm animals(e.g., cattle) via genome engineering for the gain or loss of gene functions is an important undertaking. In the initial stages of genome engineering, DNA micro-injection into ...
关键词：CATTLE CRISPR-Cas9 GENOME engineering TECHNOLOGIES TRANSGENESIS TRANSPOSON 
The genetic program Of oocytes can be modified in vivo in the zebrafish ovary被引量：1
《Journal of Molecular Cell Biology》2018年第6期479-493,共15页Xiaotong Wu Weimin Shen Bingjie Zhang Anming Meng 
the National Natural Science Foundation of China (31330052 and 31590832).
Oocytes,the irreplaceable gametes for generating a new organism,are matured in the ovary of living female animals.It is unknown whether any genetic manipulations can be applied to immature oocytes inside the living ov...
关键词：OOCYTES MICROINJECTION gene editing TRANSGENESIS MORPHOLINO ZEBRAFISH 
Primordial germ cell-mediated transgenesis and genome editing in birds被引量：2
《Journal of Animal Science and Biotechnology》2018年第2期257-267,共11页Jae Yong Han Young Hyun Park 
supported by a National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIP)(No.2015R1A3A2033826)
Transgenesis and genome editing in birds are based on a unique germline transmission system using primordial germ cells(PGCs), which is quite different from the mammalian transgenic and genome editing system. PGCs are...
关键词：AVIAN GENOME EDITING Primordial GERM cell TRANSGENESIS 
Genome editing in Drosophila melanogaster： from basic genome engineering to the multipurpose CRISPR-Cas9 system被引量：5
《Science China(Life Sciences)》2017年第5期476-489,共14页Xingjie Ren Kristof Holsteens Haiyi Li Jin Sun Yifan Zhang Lu-Ping Liu Qingfei Liu Jian-Quan Ni 
supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of the People’s Republic of China (2015BAI09B03, 2016YFE0113700);the National Natural Science Foundation of China (31371496, 31571320);the National Basic Research Program (2013CB35102)
Nowadays, genome editing tools are indispensable for studying gene function in order to increase our knowledge of biochemical processes and disease mechanisms. The extensive availability of mutagenesis and transgenesi...
关键词：genome editing Drosophila melanogaster MUTAGENESIS TRANSGENESIS CRISPR-Cas9 
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