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PDB-assistant:an integrated Web tool for analyzing and editing PDBs
《Frontiers of Computer Science》2025年第3期129-131,共3页Yukun ZHAO Changsen ZHOU Jianhong GAN Zhi-Xiong XIAO Jianbo LU Xu MA Yang CAO 
supported by the National Natural Science Foundation of China(Grant No.81973243);the National Human Genetic Resources Center,National Research Institute for Family Planning。
1 Introduction The rapidly growing protein structure data is a valuable biological resource and has been a focus of research in the academic community.The abundance of 3D structure data also presents challenges for re...
关键词：parsingpdb files biological resource d structure data biomolecules PDB format protein structure data 
The high quality genome of potherb mustard Xuecai(Brassica juncea var.multiceps)provides new insights into leaf shape variation
《Journal of Integrative Agriculture》2025年第4期1461-1476,共16页Shuangping Heng Mengdi Cui Xiaolin Li Shaoheng Zhang Guangzhi Mao Feng Xing Zhengjie Wan Jing Wen Jinxiong Shen Tingdong Fu 
supported by grants from the National Natural Science Foundation of China(32002056);the Science and Technology Research Key Project of Henan Province,China(242102111138)。
The potherb mustard Xuecai(XC)cultivar is a cruciferous vegetable that is popular either fresh or pickled.Due to the deep notches in the edges of leaves in mustard XC,this plant can be said to have multipinnately lobe...
关键词：Brassica juncea genome sequence multipinnately lobed leaves BjRCO gene editing 
Streamlined Agrobacterium rhizogenes-mediated hairy root transformation for efficient CRISPR/Cas9-based gene editing evaluation in diverse Citrullus cultivars
《Horticultural Plant Journal》2025年第2期816-826,共11页Yong Zhao Hongju Zhu Xuqiang Lu Muhammad Anees Nan He Dongdong Yang Zihao Chen Zonglie Hong Junhong Zhang Wenge Liu 
supported by the Basic Research Funds of Chinese Academy of Agricultural Sciences(Grant No.1610192023201);the National Key Research and Development Program of China(Grant No.SQ2023YFE0201239);China Agriculture Research System of MOF and MARA(Grant No.CARS-25-03);the Major Science and Technology Projects of Henan Province(Grant No.221100110400);the Funding of Joint Research on Agricultural Variety Improvement of Henan Province(Grant No.2022010503);the Agricultural Science and Technology Innovation Program(Grant No.CAAS-ASTIP-2021-ZFRI)。
The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficie...
关键词：Citrullus lanatus sgRNAs Agrobacterium rhizogenes Hairy root transformation CRISPR/Cas9 
Harnessing the Streptomyces-originating typeⅠ-E CRISPR/Cas system for efficient genome editing in Streptomyces
《Science China(Life Sciences)》2025年第4期1174-1182,共9页Yuhui Xie Xiaoyan Liu Tingting Wu Yunzi Luo 
supported by the National Key Research and Development Program of China(2018YFA0903300);the National Natural Science Foundation of China(32071426);the KeyArea Research and Development Program of Guangdong Province(2020B0303070002);the Haihe Laboratory of Sustainable Chemical Transformations(24HHWCSS00006)。
Since their discovery,CRISPR/Cas systems have significantly expanded the genetic toolbox,aiding in the exploration and enhanced production of natural products across various microbes.Among these,class 2 CRISPR/Cas sys...
关键词：genome editing type I-E CRISPR/Cas system STREPTOMYCES protospacer adjacent motif(PAM) identification natural product 
Molecular breeding of tomato:Advances and challenges
《Journal of Integrative Plant Biology》2025年第3期669-721,共53页Minmin Du Chuanlong Sun Lei Deng Ming Zhou Junming Li Yongchen Du Zhibiao Ye Sanwen Huang Tianlai Li Jingquan Yu Chang-Bao Li Chuanyou Li 
supported by the Beijing Rural Revitalization Agricultural Science and Technology Project(NY2401080000);the National Natural Science Foundation of China(31991184,32372705,32302567,32402586);the Chinese Universities Scientific Fund(15053344,15054001);the 2115 Talent Development Program of China Agricultural University;the Tai-Shan Scholars Program from the Shandong Province(TSQN202312148);the Natural Science Foundation of Shandong Province(ZR2024JQ014,ZR2024QC172);the Hainan Provincial Natural Science Foundation of China(324CXTD426);the Science and Technology Special Fund of Hainan Province(ZDYF2025XDNY082);Taishan Academy of Tomato Innovation。
The modern cultivated tomato(Solanum lycopersicum)was domesticated from Solanum pimpinellifolium native to the Andes Mountains of South America through a“two-step domestication”process.It was introduced to Europe in...
关键词：genome editing genome sequencing GERMPLASM molecular breeding TOMATO 
CRISPR/Cas genome editing for cotton precision breeding:mechanisms,advances,and prospects
《Journal of Cotton Research》2025年第1期115-128,共14页SHERI Vijay MOHAN Harikrishnan JOGAM Phanikanth ALOK Anshu ROHELA Gulab Khan ZHANG Baohong 
supported by the Cotton Incorporated.
Cotton(Gossypium hirsutum L.)is one of the most important global crops that supports the textile industry and pro-vides a living for millions of farmers.The constantly increasing demand needs a significant rise in cot...
关键词：CRISPR/Cas Biotic stress Fiber quality Genomic complexity Off-target effects Textile industry 
The Interface of Gene Editing with Regenerative Medicine
《Engineering》2025年第3期73-100,共28页Veronica E.Farag Elsie A.Devey Kam W.Leong 
supported by the National Institutes of Health(UH3NS115598).
The potential of regenerative medicine in the clinical space is vast,given its ability to repair and replace damaged tissues,restore lost functions due to age or disease,and transform personalized therapy.Traditional ...
关键词：CRISPR/Cas9 Tissue engineering Regenerative medicine Gene editing Stem cell transplantation Disease models 
Convenient,high-efficiency multiplex genome editing in autotetraploid alfalfa using endogenous U6 promoters and visual reporters
《aBIOTECH》2025年第1期81-90,共10页Xiuzhi Xia Shihao Li Na Wang Panxu Cheng Butuo Zhu Pengcheng Zhang Dahai Yang Hao Lin Lifang Niu 
supported by grants from the Biological Breeding-National Science and Technology Major Project(2022ZD04011,2023ZD04073);National Natural Science Foundation of China(32325035,32071864);Fundamental Research Funds for Central Non-profit Scientific Institution(1610392024008);Central Laboratory of Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Hainan Seed Industry Laboratory(B23CJ0208);Yunnan Academy of Tobacco Agricultural Sciences(CNTC-YC2022530000241013,CNTC110201501015(JY-02));Hohhot Key R&D Project(2023-JBGS-S-1);Inner Mongolia Center of Pratacultural Technology Innovation(CCPTZX2023B01).
CRISPR/Cas is a simple,robust,versatile tool for plant biology studies and precision plant breeding.However,establishing a high-efficiency gene editing system for multiplex editing of the autotetraploid crop alfalfa(M...
关键词：ALFALFA Hairy root system MsU6 promoter Multiplex genome editing Visual reporters 
Developing a pipeline for identification,characterization and molecular editing of cis-regulatory elements:a case study in potato
《aBIOTECH》2025年第1期91-96,共6页Min Wan Handan Xie Hongwei Guo Shenglin Jing Deying Zeng Bing Li Bo Zhu Zixian Zeng 
supported by grants from the National Natural Science Foundation of China(32170573);Sichuan Science and Technology Program(2021YFYZ0019,2023ZYD0056).
Crop breeding requires a balance of tradeoffs among key agronomic traits caused by gene pleiotropy.The molecular manipulation of genes can effectively improve target traits,but this may not reduce gene pleiotropy,pote...
关键词：Cis-regulatory element POTATO CRISPR Genome-editing 
Prospects of synthetic biology in revolutionizing microbial synthesis and drug discovery
《Life Research》2025年第1期51-60,共10页Emmanuel Chimeh Ezeako Abel Yashim Solomon Yemiode Bernard Itam Tobechukwu Christian Ezike Chinenye Peace Ogbonna Nnamdi Ginikachukwu Amuzie Emmanuel Chigozie Aham Cynthia Doowuese Aondover Gloria Oluchukwu Osuagwu Vincent Eric Ozougwu 
Synthetic biology(SynBio)is an emerging field of study with great potential in designing,engineering,and constructing new microbial synthetic cells that do not pre-exist in nature or re-engineering existing cells to a...
关键词：synthetic biology drug discovery microbial synthesis sustainable development genetic circuit gene editing 
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