Establishment of highly efficient transgenic system for black soldier fly(Hermetia illucens)  

作　　者：Zongqing Kou Xingyu Luo Yuguo Jiang Bihui Chen Yu Song Yaohui Wang Jun Xui Jeffery K.Tomberlin Yongping Huang 

机构地区：[1]Key Laboratory of Insect Developmental and Evolutionary Biology,Center for Excellence in Molecular Plant Sciences,Shanghai Insttute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai,China [2]University of Chinese Academy of Sciences,Beijing,China [3]Department of Entomology,Texas A&M University,College Station,TX,USA

出　　处：《Insect Science》2023年第4期888-900,共13页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(32021001,31830093);Strategic Priority Research Program of Chinese Academy of Sciences(Grant no.XDPB16).

摘　　要：The black soldier fly(BSF),Hermetia illucens,is a promising insect for miti-gating solid waste problems as its larvae are able to bioconvert organic waste into valuable biomass.We recently reported a high-quality genome assembly of the BSF;analysis of this genome sequence will further the understanding of insect biology and identify genes that can be manipulated to improve efficiency of bioconversion.To enable genetic manip-ulation of the BSF,we have established the first transgenic methods for this economically important insect.We cloned and identified the ubiquitous actin5C promoter(Hiactin5C-p3k)and 3 endogenous U6 promoters(HiU6:1,HiU6:2,and HiU6:3).The Hiactin5C pro-moter was used to drive expression of a hyperactive variant of the piggyBac transposase,which exhibited up to 6-fold improvement in transformation rate when compared to the wild-type transposase.Furthermore,we evaluated the 3 HiU6 promoters using this trans-genic system.HiU6:1 and HiU6:2 promoters provided the highest knockdown efficiency with RNAi and are thus promising candidates for future Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)development.Overall,our findings provide valuable genetic engineering toolkits for basic research and genetic manipulation of the BSF.

关 键 词：actin5C promoter black soldier fly hyPBase TRANSGENESIS U6 promoter 

分 类 号：Q78[生物学—分子生物学]