MICROINJECTION

作品数：56被引量：119H指数：6
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相关作者：曹小定杜莅娜吴根诚章宇朱大年更多>>
相关机构：上海医科大学复旦大学上海中医药大学华中科技大学更多>>
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相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划安徽省自然科学基金更多>>
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Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo
《Microsystems & Nanoengineering》2025年第1期403-415,共13页Xiangyu Guo Antian Zhao Youchao Zhang Huanyu Jiang Longhua Tang Bo Lu Yibin Ying Mingchuan Zhou 
support from the National Natural Science Foundation of China(32101626);the Shandong Province Key R&D Plan Project(2022LZGC020);the Natural Science Foundation of Jiangsu Province of China(BK20220490);the Fundamental Research Funds for the Central Universities under Grant No.226-2024-00227.
The microinjection of Zebrafish embryos is significant to life science and biomedical research.In this article,a novel automated system is developed for cell microinjection.A sophisticated microfluidic chip is designe...
关键词：deep learning model cell microinjectiona detect yolk center microfluidic chip microforce perception cell batch microinjection zebrafish embryo automated system 
Genome Editing in the Olive Flounder(Paralichthys olivaceus)Using CRISPR/Cas9 and a Simple Microinjection System
《Journal of Ocean University of China》2021年第6期1528-1536,共9页TAN Xungang WANG Ling WU Zhihao JIAO Shuang WANG Lijuan ZOU Yuxia JIANG Jingteng YOU Feng 
the National Natural Sci-ence Foundation of China(Nos.31672636,31772834,and 31972774);the National Key R&D Program of China(Nos.2018YFD0901202 and 2018YFD0900202);the Key Research and Development Program of Shandong Pro-vince,China(No.2019GHY1120070)。
The whole-genome sequence of the olive flounder(Paralichthys olivaceus)provides a basis for gene functional analyses,which is important for the aquaculture industry.Understanding gene function will help us to select b...
关键词：CRISPR/Cas9 gsdf MICROINJECTION myomaker needle with brake olive flounder 
Apelin-13 regulates electrical activity in the globus pallidus and induces postural changes in rats被引量：1
《Neural Regeneration Research》2021年第11期2264-2268,共5页Ying Wang Yan Xue Cui Liu Lei Chen 
supported by the National Natural Science Foundation of China,Nos.31671076(to LC),81200872(to YX);Taishan Scholars Construction Project of China(to LC).
The globus pallidus is the relay nucleus of the basal ganglia,and changes in its electrical activity can cause motor impairment.Apelin-13 is widely distributed in the central and peripheral nervous systems.It has been...
关键词：APELIN-13 basal ganglion ELECTROPHYSIOLOGY firing rate globus pallidus MICROINJECTION motor behavior movement disorder 
Egg tanning improves the efficiency of CRISPR/Cas9-mediated mutant locust production by enhancing defense ability after microinjection被引量：1
《Journal of Integrative Agriculture》2021年第10期2716-2726,共11页ZHANG Ting-ting WEN Ting-mei YUE Yang YAN Qiang DU Er-xia FAN San-hong Siegfried ROTH LI Sheng ZHANG Jian-zhen ZHANG Xue-yao ZHANG Min 
This work was supported by the National Natural Science Foundation of China(32070502,31730074,32072419 and 31501905).
The mutant efficiency and hatching ratio are two key factors that significantly affect the construction of genome-modified mutant insects.In the construction of CRISPR/Cas9-mediated dsLmRNase2^(–/–)mutant locusts,we...
关键词：brown tanned eggs CRISPR/Cas9 mutant locusts MICROINJECTION defense ability 
Comparison of gene expression responses of zebrafish larvae to Vibrio parahaemolyticus infection by static immersion and caudal vein microinjection
《Aquaculture and Fisheries》2021年第3期267-276,共10页Xinya Guo Ce Ji Xuan Du Jianfeng Ren Yao Zu Weiming Li Qinghua Zhang 
This work was supported by the Ministry of Education Returnees Research Fund(D-8002-15-0042);the China-US Ocean Research Center Fund(A1-3201-19-3013);the Shanghai First-Class Discipline Construction Fund.
infection model for studying inflammation and the innate immune response.We investigated the genes and signaling pathways activated by static immersion and caudal vein microinjection infection using transcriptome prof...
关键词：IMMERSION MICROINJECTION Transcriptome Vibrio parahaemolyticus ZEBRAFISH 
Microinjection of a growth factor cocktail affects activated microglia in the neocortex of adult rats被引量：3
《Neural Regeneration Research》2020年第9期1709-1715,共7页Ruo-Xu Liu Jie Ma Ning Guo Shao-Jun Liu 
supported by a grant from State Key Laboratory of Proteomics of China,No.SKLP-K201401(to SJL);the National Key Project of Basic Research of China,No.2009CB918301(to SJL);the National Natural Science Foundation of China,Nos.30430310,30140001,30370460(to SJL)
Microglia, as the resident immune cells in the central nervous system, play important roles in regulating neuronal processes, such as neural excitability, synaptic activity, and apoptotic cell clearance. Growth factor...
关键词：adult neocortex CD68 IBA1 MICROINJECTION PHAGOCYTE selective phagocytosis ultrastructure 
Delivery methods for CRISPR/Cas9 gene editing in crustaceans被引量：1
《Marine Life Science & Technology》2020年第1期1-5,共5页Sen Xu Thinh Phu Pham Swatantra Neupane 
NIH Grant R35GM133730 to Sen Xu.
In this mini-review,we provide an up-to-date overview of the delivery methods that have been used for CRISPR/Cas9 genomic editing in crustacean species.With embryonic microinjection as the main workforce for deliverin...
关键词：MICROINJECTION ELECTROPORATION VITELLOGENESIS DAPHNIA Parhyale hawaiensis Exopalaemon carinicauda 
Screening and evaluation of antioxidants from lees by micro-injector systems combined with a fluorescent probe,N-borylbenzyloxycarbonyl-3,7-dihydroxyphenoxazine,in living Drosophila被引量：2
《Chinese Chemical Letters》2018年第10期1521-1527,共7页Guangcui Chu Dandan Cheng Wenjuan Liu Xuefeng Wang Xiaotao Hou Yuanyuan Hou Hongjun Wei Jiagang Deng Gang Bai 
financially supported by the Collaborative Innovation Center of Research on Functional Ingredients from Agricultural Residues (Guangxi University of Chinese medicine,No. CICAR 2015-B2)
Over the past few decades, the determination of antioxidant activity by chemical probe has been widely reported, but in vivo evaluation via model organisms of Drosophila melanogaster and rapid discovery system has not...
关键词：LEES Antioxidant MICROINJECTION HPLC-UV-FLD NBCD PHPAA 
The genetic program Of oocytes can be modified in vivo in the zebrafish ovary被引量：1
《Journal of Molecular Cell Biology》2018年第6期479-493,共15页Xiaotong Wu Weimin Shen Bingjie Zhang Anming Meng 
the National Natural Science Foundation of China (31330052 and 31590832).
Oocytes,the irreplaceable gametes for generating a new organism,are matured in the ovary of living female animals.It is unknown whether any genetic manipulations can be applied to immature oocytes inside the living ov...
关键词：OOCYTES MICROINJECTION gene editing TRANSGENESIS MORPHOLINO ZEBRAFISH 
Expression of pBac 3xP3 EGFP and pBac A3 EGFP Vector in Transgenic Spodoptera litura Fabricius
《Trends in Genetics and Evolution》2018年第1期1-6,共6页Zhongling Gong Wenjie Xu 
In this study, the actin A3 promoter was inserted into piggyBac and constructed into pBacA3, which was inserted into piggyBac. The promoter was composed of the promoter and nerve-specific 3xP3 promoter, the enhanced g...
关键词：SPODOPTERA litura MICROINJECTION pBacA3EGFP pBac3xP3 EGFP FLUORESCENT EXPRESSION 
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