长链非编码RNA UCA1在胃癌多药耐药中的作用研究  

The Role of lnc RNA UCA1 on Drug Resistance in Gastric Cancer

作　　者：张哲[1] 郭昊[1] 董加强[1] 蒋孝明[1,2] 聂勇战[1] 樊代明[1] 

机构地区：[1]第四军医大学西京消化病医院肿瘤生物学国家重点实验室,陕西西安710032 [2]宁波大学医学院生物化学与分子生物学研究所浙江省病理生理学技术研究重点实验室,浙江宁波315211

出　　处：《现代生物医学进展》2015年第15期2811-2814,2819,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金重点项目(81030044)

摘　　要：目的:研究胃癌耐药细胞及其亲本细胞中长链非编码RNA UCA1的表达差异,探讨UCA1在胃癌多药耐药中的作用。方法:通过实时荧光定量PCR(q RT-PCR)检测胃癌耐药细胞SGC7901/ADR、SGC7901/VCR及其亲本细胞SGC7901中UCA1的表达差异;通过si RNA转染降低SGC7901/ADR中UCA1表达,MTT法检测细胞半数抑制浓度(IC50)的变化,流式细胞仪检测细胞凋亡变化。结果:QRT-PCR结果显示,UCA1在SGC7901/ADR和SGC7901/VCR胃癌耐药细胞表达显著高于SGC7901胃癌亲本细胞;MTT实验表明,干扰UCA1的SGC7901/ADR相对于阴性对照(NC)组的IC50显著降低;凋亡检测结果显示,在相同剂量化疗药物作用下,干扰UCA1后SGC7901/ADR凋亡率显著高于NC组;Western blot证实,干扰UCA1表达可显著降低BCL-2蛋白表达。结论:长链非编码RNA UCA1在胃癌耐药细胞表达显著升高,干扰UCA1表达可明显逆转胃癌耐药,UCA1可作为治疗胃癌耐药的重要分子靶标。Objective： To investigate the differential expression of IncRNA UCA1 in SGC7901/ADR, SGC7901NCR and SGC7901 gastric cancer cell lines and to explore its role in multi-drug resistance （MDR） of gastric cancer （GC）. Methods： The expression level of UCA1 in SCJC7901/ADR, SGC7901/VCR and SGC7901 cell lines was obtained by quantitative real-time PCR （qRT-PCR） detec- tion. SiRNA transfection was applied to knockdown UCA1 expression in SGC7901/ADR. The drug sensitivity （IC50） of SGC7901/ADR were detected by MTT assay; apoptosis rates were detected by flow cytometry and the protein expression of Bcl-2 by western blot. Results： QRT-PCR results showed that the expression level of UCA1 in SGC7901/ADR and SGC7901/VCR was significantly higher than that in parental SGC7901 cells. The MTT assay and flow cytometry analysis showed that the drug sensitivity （IC50） and apoptosis rates of SGC7901/ADR were significantly increased compared with negative control （NC） group. Western blot results confirmed that knockdown of UCA1 in SGC7901/ADR can significantly reduce the expression of BCL-2 protein. Conclusions： Long non-coding RNA UCA1 was signifieantly up-regulated in two MDR GC cell sublines, SGC7901/ADR and SGC7901/VCR. Our findings indicate that UCA1 plays a positive role in the regulation of the MDR phenotype ofGC MDR cell sublines and is a potential target to resverse GC MDR.

关 键 词：长链非编码RNA UCA1 胃癌 多药耐药 

分 类 号：R573[医药卫生—消化系统]