急性肝衰竭大鼠肝脏的蛋白质组学研究  

Proteomics study on liver of acute hepatic failure rats

作　　者：蔡磊[1] 侯外林[2] 程远[1] 潘明新[1] 高毅[1] 

机构地区：[1]南方医科大学珠江医院肝胆二科,广东广州510280 [2]郴州市第一人民医院肝胆外科,湖南郴州423000

出　　处：《重庆医学》2015年第12期1592-1595,共4页Chongqing medicine

基　　金：国家高技术研究发展计划(863计划)课题(2006AA02A141);广东省科技计划项目(2011B031800127)

摘　　要：目的筛选急性药物性肝衰竭大鼠肝脏组织的差异表达蛋白质。方法将24只SD大鼠分为两组,12只通过腹腔注射10g/L的D-氨基半乳糖建立大鼠急性肝衰竭模型(实验组),12只腹腔注射生理盐水作为对照组。提取两组大鼠肝脏组织的蛋白质,定量后进行IEF和十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)双向凝胶电泳(2-DE)分离,通过软件找到差异蛋白点并使用基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF-MS)进行鉴定。结果成功鉴定出27个有效的差异蛋白质点,其中实验组较对照组上调15个,下调12个。结论急性药物性肝衰竭模型大鼠肝脏酪蛋白激酶Iα(CKⅠα)、酪氨酸蛋白激酶(PTK)、增殖细胞核抗原(PCNA)等蛋白的表达较正常大鼠存在显著差异。Objective To screen the differentially expressed proteins in the liver tissue of the drug-induced acute hepatic fail- ure rats. Methods Twenty-four male SD rats were randomly divided into two groups,the experimental group （12 cases） was given D-galactosaminel0g/L by intraperitoneal injection and the control group（12 cases） was given normal saline by intraperitoneal injec- tion. The total proteins in the liver tissue samples were extracted,quantitated,and subjected to separate by the two-dimension elec- trophoresis（2-DE） of isoelectric focusing（IEF） and SDS-PAGE, found out the discrepant protein spots by the software and per- formed the identification by MALDI-TOF-MS. Results 27 differential protein spots were successfully identified,and 15 up-regula- ted and 12 down-regulated proteins expressions were obtained in the experimental group compared with the control group. Conclu- sion The significant differences in the expressions of proteins, such as casein kinase I（CK I α）, tyrosine protein kinase（PTK）, pro- liferating cell nuclear antigen（PCNA） ,etc. in the liver exist between the acute hepatic failure model rats and the normal ones.

关 键 词：大鼠 肝功能衰竭 急性 蛋白质组学 电泳 凝胶 双相 光谱法 质量 基质辅助激光解吸电离 

分 类 号：R575.3[医药卫生—消化系统]