缝隙连接蛋白pannexin1在神经病理性疼痛大鼠脊髓背角的表达变化  

Change of pannexin1 expression in dorsal horn of spinal cord in rats with neuropathic pain

作　　者：周功锐 包晓航[1] 毛庆祥[1] 龙宗泓 景胜[1] 黄静[1] 杨天德[1] 

机构地区：[1]第三军医大学新桥医院麻醉科,重庆400037

出　　处：《重庆医学》2015年第12期1596-1598,1602,共4页Chongqing medicine

摘　　要：目的观察缝隙连接蛋白pannexin1(PX1)在坐骨神经分支选择结扎模型大鼠脊髓背角上的表达变化。方法健康SD雄性大鼠50只,分为对照组(WT组,n=10)、假手术组(sham组,n=10)和坐骨神经分支选择性损伤组(SNI组,n=30)。SNI组20只大鼠于术后3、5、7、14d(n=5),WT、sham组于术后14d(n=5)取脊髓腰段用Western blot法检测PX1表达变化,另20只大鼠于术后7d取脊髓腰段进行免疫组化染色,检测脊髓背角内胶质纤维酸性蛋白(GFAP)的表达水平。SNI组中10只于建模前进行鞘内置管,术后7d分别向鞘内注射生理盐水20μL(SNI+NS组)或甘珀酸(CBX)20μL(SNI+CBX组),再进行免疫组化染色。结果 SNI组脊髓背角内PX1表达增多,并随时间增强,明显高于WT、sham组(P<0.05);7d后SNI组脊髓背角内GFAP表达较WT、sham组明显增强(P<0.05);SNI+CBX组GFAP表达较SNI+NS组明显下调(P<0.05)。WT、sham组脊髓背角的PX1蛋白和GFAP表达差异无统计学意义(P>0.05)。结论缝隙连接蛋白PX1可能参与了星形胶质细胞的激活,提示其在因外周神经损伤引起的神经病理性痛中起重要作用。Objective To observe the expression of pannexinl（PXl） in the dorsal horn of spinal cord in model rats with neu- ropathic pain after selective ligation of sciatic nerve branches. Methods 50 male SD rats were randomly divided into 3 groups,inclu- ding the control group（WT group, n= 10）, sham operation group（sham group, n= 10） and sciatic nerve branch selective injury group（SNI group, n= 30）. 30 rats were killed on postoperative 3,5,7,14 d and the lumbar segment of the spinal cord was taken for detecting the expression of PX1 by using Western blot. Other 20 rats were killed on 7 d after SNI and the expression of glial fibril- lary acidic protein（GFAP） in the spinal cord was detected with immunohistotogy. Among them, 10 rats in the SNI group were trea- ted with intrathecal intubation before operation and administrated with saline 20 μL or carbenoxolone（CBX） 20 μL by intrathecal injection on postoperative 7 d for determining the expression of GFAP by the immunohistology. Results The expression of PX1 in the SNI group was increased and enhanced with time, which was significantly higher than that in the WT group and the sham group （P〈0.05） ; the GFAP expression on 7 d in the SNI group was obviously increased compared with the WT group and the sham group（P〈0.05） ;after intrathecal injection of CBX,the expression of GFAP was significantly decreased compared with that in the normal saline group（P〈0.05）. No statistically significant differences in the expression of PX1 and GFAP were found in the WT group and the sham group. Conclusion PX1 may be involved in the activation of astrocytes,prompting that PX1 plays an important role in the neuropathic pain caused by the peripheral nervel injury.

关 键 词：神经病理性疼痛 缝隙连接类 Pannexin1 星形胶质细胞 甘珀酸 胶质纤维酸性蛋白 

分 类 号：R741[医药卫生—神经病学与精神病学]