Dkk-1及Bcl-2蛋白在人鼻腔鼻窦鳞状细胞癌中的表达及意义  

Expression and significance of Dkk-1 and Bcl-2 protein in sinonasal squamous cell carcinoma

作　　者：陆鸿略[1] 张淑君[1] 岳文慧[1] 康菲[2] 郝然[1] 

机构地区：[1]承德医学院附属医院耳鼻咽喉科,河北承德067000 [2]承德医学院附属医院体检科,河北承德067000

出　　处：《重庆医学》2015年第12期1652-1654,1657,共4页Chongqing medicine

摘　　要：目的探讨Dickkopf-1(DKK-1)及B细胞淋巴瘤/白血病-2(B cell lymphoma/lewkmia-2,Bcl-2)蛋白在人鼻腔、鼻窦鳞状细胞癌(SNSCC)中的表达及其临床意义。方法采用免疫组化SP法及Western blot技术分别检测30例SNSCC(SNSCC组),38例鼻腔、鼻窦内翻性乳头状瘤(SNIP组)以及20例中鼻甲黏膜组织(对照组)中DKK-1和Bcl-2蛋白的表达情况。结果与SNIP组及对照组比较,DKK-1蛋白在SNSCC组中的表达明显下调,Bcl-2蛋白在SNSCC组中的表达明显上调;在SNSCC组中,DKK-1及Bcl-2蛋白在高、中分化与低分化组中的阳性表达率分别为100.00%、68.75%、33.33%及50.00%、62.50%、100.00%,均差异有统计学意义(P<0.05)。结论 DKK-1蛋白可能在SNSCC的发生和发展过程中起着重要的促进作用,且与Bcl-2蛋白呈负相关,DKK-1蛋白可能成为SNSCC基因治疗的新靶点。Objective To explore the expression and significance of Dickkopf-1 （Dkk-1） and B cell lymphoma/leukemia-2 （Bcl-2） protein in sinonasal squamous cell carcinoma（SNSCC）. Methods The immunohistochemical SP method and Western blot method were adopted to determine the expression of Dkk-1 and Bcl-2 in 30 specimens of SNSCC（SNSCC group）, 38 specimens of sinonasal inverted papillomas（SNIP group） and 20 specimens of middle turbinate mucosa（control group）. Results The expression of DKK-1 protein in the SNSCC group was significantly down-regulated compared with the SNIP group and the control group,while the expression of Bcl-2 protein in the SNSCC group was significantly up-regulated compared with the SNIP group and the control group;in the SNSCC group,the positive rates of DKK-1 protein and Bcl-2 protein in the high and middle differentiation group and the low differentiation group were 100.00% ,68.75% ,33.33% and 50.00% ,62.50% ,100.00% respectively,the differences were statistically significant （P^0.05）. Conclusion Dkk-1 protein may play an important promoting role in the development and pro- gression process of SNSCC, the expression of Dkk-1 protein has a negative correlation with the expression of Bcl-2 protein in SNSCC,which may become a new target spot of SNSCC gene therapy.

关 键 词：鼻腔肿瘤 鼻窦肿瘤 肿瘤 鳞状细胞 DKK-1蛋白 BCL-2蛋白 免疫组织化学 

分 类 号：R739.62[医药卫生—肿瘤]