不同品种、产地瓜蒌皮中游离氨基酸类成分指纹图谱研究  

Fingerprints of Amino Acid of Trichosanthes Pericarpium from Different Varieties and Habitats by HPLC

作　　者：郝变 潘丽丽[1] 袁少雄[1] 杨颂[1] 李亭亭[1] 徐何方 李向日[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中医药学报》2015年第2期14-18,共5页Acta Chinese Medicine and Pharmacology

基　　金：保健食品中违禁物质检测技术研究(2012BAK08B02)

摘　　要：目的:建立瓜蒌皮中游离氨基酸类成分指纹图谱,并比较研究不同品种、产地瓜蒌皮氨基酸含量差异,为科学评价与有效控制瓜蒌皮质量提供依据。方法:采用异硫氰酸苯酯(PITC)为衍生试剂,色谱柱为Eclipse XDB C18(4.6mm×250mm,5μm);流动相A:乙腈-水(4∶1),流动相B:醋酸钠-乙腈溶液(93∶7),梯度洗脱;流速:1m L/min;检测波长:254nm;柱温为30℃。结果:建立了瓜蒌皮中19种游离氨基酸类成分液相指纹图谱共有模式,并对不同产地瓜蒌皮药材进行了相似度比较和聚类分析。结论:本方法简便、快速、重复性好,可用于鉴别和区分不同来源的瓜蒌皮药材,为全面控制瓜蒌皮药材的质量提供依据。Objective：To set up fingerprints of amino acid of trichosanthes pericarpium from different varieties and habitats by HPLC,in order to establish a sensitive and specific method for controlling the quality of trichosanthes pericarpium. Methods：Phenyl isothiocyanate （ PITC） was used as a derivatization reagent.Chromatographic condition：Eclipse XDB C18 of HPLC column, （4.6mm ×250mm,5μm）;mobile phase：gradient elution of acetonitrile-water （4∶1） （A） and sodium acetate solution-acetonitrile （93∶7） （B）, detector wavelength：254nm;column temperature：30℃at the flow rate of 1mL/min.Results：The common modes of HPLC fingerprints of 19 kinds of amino acid in trichosanthes pericarpi-um were set up and the similar degrees to the crude drugs of different varieties and habitats were compared .Conclusion：The method is accurate, reliable and sensitive, which can be used as a quality control item for trichosanthes pericarpium collected from different sources and varieties.

关 键 词：异硫氰酸苯酯 瓜蒌皮 游离氨基酸 高效液相 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]