莪术醇对人脑胶质瘤U251细胞增生与凋亡影响的实验研究  

Effect experimentation of curcumol on proliferation and apoptosis of human glioma U251 cell

作　　者：陈珮[1] 秦铁城[1] 王伟刚[1] 

机构地区：[1]中国石油天然气集团公司中心医院神经外科,廊坊065000

出　　处：《国际外科学杂志》2015年第3期186-190,F0003,共6页International Journal of Surgery

摘　　要：目的本研究观察中药活性成分莪术醇对人脑胶质瘤细胞株U251增生与凋亡的影响，检测其对胶质瘤细胞凋亡相关基因表达的影响，初步探讨其抗胶质瘤的可能机制，为脑胶质瘤的中草药治疗积累实验数据。方法以体外培养的人脑胶质瘤细胞株U251为模型，以培养基稀释莪术醇成不同浓度，观察与检测以下内容：MTr法检测不同时间、不同浓度莪术醇对U251细胞增生的影响；显微镜观察不同浓度莪术醇对U251细胞形态学的影响；流式细胞仪检测不同浓度莪术醇对U251细胞凋亡的影响；RT—PCR法检测不同浓度莪术醇对U251细胞中凋亡相关基因bel—2与COX-2表达水平的影响。结果莪术醇对人脑胶质瘤U251细胞增生有抑制效应，且表现出浓度依赖性与时间依赖性（P〈0．05）。结论莪术醇对人脑胶质瘤U251细胞有明确的增生抑制作用。诱导凋亡、抑制增生可能为莪术醇抑制胶质瘤增生效应的重要机制。莪术醇下调人脑胶质瘤U251细胞中bel-2与COX-2的基因表达水平可能为其诱导凋亡、抑制增生的重要机制之一。Objective To observe the effect of the active ingredient present study curcumol impact on human glioma cell line U251 proliferation and apoptosis, its effect on the detection of apoptosis-related gene expression in glioma, and explore the possible mechanism of its anti-glioma, Chinese herbal medicine for the treatment of glioma accumulation of experimental data. Methods In cultured human glioma cell line U251 as a model to medium dilu- tion Curcumol different concentrations, observation and detection following： MTF assay at different times, different concentrations curcumol on U251 cell proliferation; microscope Effect of different concentrations of curcumol U251 cell morphology; ＂affected by flow cytometry curcumol different concentrations on apoptosis in U251 ; RT-PCR meth- od to detect different concentrations curcumol on U25l cell apoptosis-related gene bcl-2 and COX-2 expression lev- els of influence. Results Curcumol U251 human glioma cell proliferation inhibition effect and showed concentra- tion- dependent and time-dependent manner （P 〈 0.05）. Conclusions Curcumol U251 human glioma cell prolif- eration clear. Induce apoptosis and inhibit proliferation of glioma should be an important mechanism of inhibition of proliferation effect of curcumol. Curcumol down on U251 cells bcl-2 gene expression levels and COX- 2should be its induction of apoptosis, an important mechanism of inhibition of proliferation.

关 键 词：细胞凋亡 神经胶质瘤 基因 BCL-2 COX-2 莪术醇 

分 类 号：R730.264[医药卫生—肿瘤]