poly(dA:dT)促进体外培养的人绒毛组织AIM2炎性体活化  

poly(dA:dT) Actives AIM2 Inflammasome in Human Placental Villi in Vitro

作　　者：周敏[1] 李宁[1] 付艳[2] 杜崇涛[1] 陈巍[1] 杨勇军[1] 

机构地区：[1]吉林大学动物医学学院兽医微生物与免疫学实验室,吉林长春130062 [2]吉林大学第一医院,吉林长春130021

出　　处：《现代生物医学进展》2015年第14期2614-2616,2632,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31172298;31372410);长江学者和创新团队发展计划(IRT1248)

摘　　要：目的:在妊娠过程中,胎盘可能暴露于多种病原微生物,威胁胎儿正常生长发育。为探讨人胎盘绒毛组织是否表达AIM2炎性体成员基因以及人胎盘组织的AIM2炎性体的活化形式。方法:以THP-1细胞来源的RNA和蛋白作为阳性对照,分别应用RT-PCR和Western blot方法检测人早孕期胎盘绒毛组织中AIM2炎性体两个相关基因AIM2和ASC的表达。分离和体外培养人胎盘绒毛膜组织,并用不同浓度的poly(d A:d T)进行转染,处理24小时后,分别收集组织培养上清和蛋白裂解液,Western blot检测蛋白裂解液中caspase-1的活化,ELISA检测培养上清中IL-1β的分泌。结果:RT-PCR和Western blot结果均显示人早孕期胎盘绒毛组织组成性表达AIM2炎性体相关基因AIM2和ASC。同时,体外培养的人胎盘绒毛组织在转染5μg/m L poly(d A:d T)后,caspase-1剪切片段p10显著增多,培养上清中IL-1β分泌也显著增多(P<0.01)。结论:人胎盘绒毛组织存在功能性的AIM2炎性体,能够被胞内双链DNA活化。Objective： During pregnancy, the placenta may become exposed to microorganisms, which may pose a potential threat to the embryo/fetus well-being. This study was preformed to investigate expression and function of the AIM2 inflammasome in human placental villi. Methods： The expression of AIM2 and ASC, two components of the AIM2 inflammasome, were determined in human placental villi by RT-PCR and Western blot. RNA and protein from THP-1 cells were used as positive control. Human placental villi were isolated and cultured in vitro, then transfected with different doses ofpoly （dA：dT） for 24 hours. The supernatant were collected for analysis of 1L-1β secretion and tissue lysates were harvested for detection of caspase-1 activation by Western blot. Results： RT-PCR and Western blot analysis showed that ASC and AIM2 were constitutively expressed in human placental villi. Furthermore, treatment with 5 μg/mL poly （dA：dT） markedly induced IL-1β release and increased caspase-1 activity. Conclusion： The AIM2 inflammasome is expressed and may plays an important role in human placental villi, which is activated by cytosolic double-stranded DNA stimulation.

关 键 词：人胎盘绒毛组织 AIM2炎性体 CASPASE-1 IL-1β 

分 类 号：Q132.7[生物学—普通生物学] R-33[医药卫生]