珍贵束丝放线菌固体发酵生产安丝菌素及安丝菌素P-0制备  

Solid-state fermentation of Actinosynnema pretiosum ATCC 31565 for ansamitocins and preparation of ansamitocin P-0

作　　者：南艳妮 白晓光[1] 孙桂芝[1] 许乐幸[1] 王玉成[1] 武临专[1] 

机构地区：[1]卫生部抗生素生物工程重点实验室中国医学科学院/北京协和医学院医药生物技术研究所,北京100050

出　　处：《中国抗生素杂志》2015年第4期241-244,273,共5页Chinese Journal of Antibiotics

基　　金："重大新药创制"科技重大专项(No.2012ZX09301002-001-016);中国医学科学院医药生物技术研究所中央级公益性科研院所基本科研业务专项(No.IMBF201302)

摘　　要：目的获得安丝菌素及安丝菌素P-0纯品。方法珍贵束丝放线菌ATCC 31565经固体发酵培养,培养物乙酸乙酯萃取、硅胶柱层析后得到安丝菌素P-2、P-3和P-4混合物。安丝菌素混合物经化学法脱去C-3酰基侧链,采用制备型HPLC获得安丝菌素P-0纯品。结果每升固体发酵培养基可获得安丝菌素P-2、P-3和P-4混合物(98±2)mg(纯度85%)。50mg该安丝菌素样品可获得安丝菌素P-0纯品约31mg(纯度大于99%),产率约82%。结论将安丝菌素固体发酵培养与提取、去酰基化和HPLC纯化相结合,建立了一套安丝菌素P-0纯品的实验室制备工艺。Objective To produce ansamitocins （a mixture of P-2, P-3 and P-4） by Actinosynnema pretiosum ATCC 31565 and obtain ansamitocin P-0 by chemical de-acylation of ansamitocins. Methods Solid-state fermentation of A. pretiosum ATCC 31565 was carried out for ansamitocins production. Ethyl acetate extraction andsilica gel column fractionation were conducted to obtain the mixture preparation of ansamitocins P-2, P-3 and P-4. The mixture was then used to generate ansamitocin P-0 by chemical de-acylation. Results Mixture of ansamitocins （purity ≥85% by HPLC） was obtained at a yield of （98±2）mg/L. The yield of ansamitocin P-0 （purity ≥ 99% by HPLC） was about 82% from ansamitocins. Conclusion A simple and efficient procedure for laboratory production of ansamitocins and ansamitocin P-0 was established.

关 键 词：安丝菌素P-0 珍贵束丝放线菌ATCC31565 固体发酵 

分 类 号：Q939.9[生物学—微生物学]