低温肠内喂养对胃缺血再灌注损伤大鼠影响的实验研究  

Experimental study on influence of low temperature enteral feeding on rats with gastric ischemia-reperfusion injury

作　　者：戴利[1] 罗利娜[1] 曾秋霞[1] 

机构地区：[1]广东省深圳市宝安区人民医院

出　　处：《护理研究（上旬版）》2015年第5期1575-1577,共3页Chinese Nursing Researsh

摘　　要：[目的]探讨低温肠内喂养对胃缺血-再灌注损伤(GI-RI)大鼠影响及主要作用机制。[方法]将40只雄性SD大鼠随机分为肠内喂养温度10℃、24℃、32℃、40℃四组,采用夹闭腹腔动脉30min造成大鼠胃缺血,松开动脉夹后再灌注形成胃缺血-再灌注损伤模型,手术后2h开始给予肠内营养(EN),术后48h观察胃黏膜损伤指数,采用免疫组织化学方法检测肿瘤坏死因子-α(TNF-α)在胃黏膜细胞中的表达,采用ELISA法检测血清TNF-α含量。[结果]10℃组肠内喂养胃黏膜损伤指数明显低于24℃组(P<0.05)、32℃组(P<0.01)、40℃组(P<0.01),胃黏膜TNF-α阳性表达低于24℃组(P<0.05)、32℃组(P<0.05)、40℃组(P<0.01);胃黏膜损伤指数、TNF-α阳性表达与肠内喂养温度均呈正相关(P<0.01);各组大鼠血清TNF-α含量差异无统计学意义(P>0.05)。[结论]10℃肠内喂养温度对大鼠因缺血再灌注造成的急性胃黏膜损伤具有保护作用,其机制可能与低温抑制机体过度表达的炎症反应有关。Objective:To probe into the influence of low temperature enteral feeding on rats with gastric ischemia reperfusion injury (GI RI)and its main mechanisms.Methods:A total of 40 male Sprague Dawley(SD)rats were randomly divided into four groups according to enteral feeding temperature:10 ℃,24 ℃,32 ℃ and 40 ℃(n=10 per group).Clamping the celiac artery for 30 min to lead gastric ischemia and then to removed the clip to make GI RI model.At 2 h later after reperfusion,all rats received enteral feeding .When 48 h later after the reperfu-sion,gastric mucosal injury index was counted grossly.The expression of tumor necrosis factor α(TNF α)of gastric mucosal cell was detected by immunohistochemistry.And the plasma TNF a was detected with enzyme linked immunosorbent assay(ELISA).Results:The gastric mucosal injury index and positive expression of TNF αin 10 ℃ group were significantly lower than that in the other groups(P <0.05 or P <0.01).Meanwhile,those were positively correlated with enteral feeding temperature(P <0.01 ).There was no statistically difference in terms of plasma TNF α content among four groups(P >0.05).Conclusion:The results suggested that 10 ℃enteral feeding show a gastroprotective effect for the ischemia reperfusion injury in rats,The mechanism may be related to low temperature inhibiting the body’s inflammatory response over expression.

关 键 词：肠内喂养 温度 大鼠 胃缺血再灌注 急性胃损伤 

分 类 号：R573[医药卫生—消化系统]