离水操作胁迫对红鳍东方鲀血清生化指标的影响  

Effects of Handling Stress out of Water on Serum Parameters in Tiger Puffer Takifugu rubripes

作　　者：程民杰[1] 黄亚东[1,2] 徐文敏 万刚涛 邢克智[1] 陈成勋[1] 王庆奎[1] 郭永军[1] 孙学亮[1] 

机构地区：[1]天津农学院水产学院,天津市水产生态与养殖重点实验室,天津300384 [2]天津海升水产养殖有限公司,天津300272 [3]天津市立达海水资源开发有限公司,天津300280

出　　处：《水产科学》2015年第4期227-231,共5页Fisheries Science

基　　金：国家“十二五”科技支撑计划项目(2011BAD13B07);国家星火重大项目(2012GA610003);天津市重大种业专项(12ZCDZNC05900)

摘　　要：在水温（22．0±0．3）℃条件下，对红鳍东方鲀[体质量（181．88±14．95）g]进行离水操作（离水模拟挑鱼动作3 min ，放入水中2 min ，再离水操作3 min）。操作后0、4、8、12、18、24、36、48、72、96、120、168 h取样，测定血清指标，评价离水操作对红鳍东方鲀的影响。试验结果表明，转氨酶（谷丙转氨酶、谷草转氨酶）在离水操作后升高，谷丙转氨酶在72 h后恢复正常，谷草转氨酶在8 h后恢复至对照水平；抗氧化酶（总超氧化物歧化酶、过氧化氢酶、总抗氧化酶）均呈现先升后降趋势，总超氧化物歧化酶在12 h后恢复至对照水平；过氧化氢酶在18 h达到最大，48 h恢复正常，总抗氧化酶在8～24 h时显著高于对照组（P＜0．05）。丙二醛含量持续高于对照组；肌酐和尿素氮含量在离水操作后升高；碱性磷酸酶在8 h时显著低于对照组（P＜0．05），之后恢复至对照水平。总蛋白含量胁迫组与对照组差异不显著（P＞0．05）。各项指标显示红鳍东方鲀的肝脏和肾脏可能受到了损伤，鱼体内的平衡机制被打破，代谢出现紊乱。在胁迫后72 h鱼体恢复正常。The serum indices were determined 0 ,4 ,8 ,12 ,18 ,24 ,36 ,48 ,72 ,96 ,120 ,and 168 h after handling out of water in tiger puffer Taki f ugu rubripes with body weight of （181 .88 ± 14 .95） g handled out of water （out of water for 3 min ,in water for 2 min ,and then out of water for 3 min ） at water temperature of （22 .0 ± 0 .3） ℃ .The results showed that activities of glutamate‐pyruvate transaminase （GPT） and glutamate‐oxaloacetate transaminase （GOT） were found to be increased after handling ,GPT and GOT being recovery to the control level 72 h and 8 h later ,respectively .The activities of superoxide dismutase （SOD） and catalase （CAT） were increased first and then decreased gradually ,being recovery to the control level 12 h later .The maximal CAT activity was observed 18 h after handling ,and returned to the control level after 48 h ,significantly higher than that in the control group from 8 h to 24 h later （P〈0 .05） .There was higher content of malonaldehyde （MDA） in the fish in the treatment group than in the control group during the test .The levels of creatinine （Cr） and urea nitrogen （BUN） were found to be increased after handling ,and then returned to the control level . The activity of alkaline phosphatase （AKP） was found to be significantly lower than that in the control group after handling （P〈0 .05） ,AKP being recovery to the control level .There was no significant difference in content of total protein （TP） between the handling group and the control group（ P〉0 .05） .The findings indicate that the handling out of water might damage liver and kidney due to unbalancing and disorder .The handled fish will returned to the normal level 72 h after handling .

关 键 词：红鳍东方鲀 离水操作 胁迫 血清 

分 类 号：S965.339[农业科学—水产养殖]