琯溪蜜柚CmPME1的克隆及其在果实发育过程中的表达分析  

Cloning and Expression Analysis of CmPME1 During Fruit Development of Citrus maxima(Burm.) Merr.

作　　者：刘志发[1,2] 潘腾飞[1,2] 潘东明[1,2] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学园艺产品贮藏保鲜研究所,福建福州350002

出　　处：《热带作物学报》2015年第4期687-691,共5页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划项目"台湾农业新品种;新技术引进创新研究与示范"(No.2007BAD07B01);福建省种业创新与产业化工程项目;福建农林大学创新团队项目"园艺植物种质与高优生产技术创新"(No.cxtd12013)

摘　　要：利用RT-PCR技术从‘琯溪蜜柚’中克隆得到1条PME(pectinmethylesterase)基因的ORF序列,命名为Cm PME1。该序列编码一个含有582个氨基酸的蛋白质,分子量63.37 ku,该蛋白属于稳定的碱性亲水性蛋白。同源性分析表明:其编码的氨基酸序列与可可树(Theobroma cacao)、毛果杨(Populus trichocarpa)的同源性分别为76%、74%,与甜橙的氨基酸序列同源性高达99%。实时荧光定量PCR结果表明,随着果实的发育,Cm PME1的表达量逐渐升高,在花后170 d达到最高,然后逐渐降低,远中柱汁胞Cm PME1的表达量高于近中柱。The ORF of PME gene was cloned from Citrus maxima（Burm.） Merr. by RT-PCR, and it was named as Cm PME1. The Cm PME1 encods a protein with 582 amino acids. The deduced protein molecular weight was63.37 ku and it was a stable alkaline hydrophilic protein. The deduced amino acid sequence of Cm PME1 showed high identity with that of other plants, for instance Cm PME1 shared 76% homology with PME protein of Theobroma cacao, 74% homology with PME protein of Populus trichocarpa and 99% homology with PME protein of Citrus sinensis. The result of real-time fluorescence quantitative PCR analysis showed during fruit development,Cm PME1 expression level rose at first, and reached the highest amount at 170 days after flowering, then reduced gradually. The study also indicated the expression level of Cm PME1 in juice sac away from the column was higher than in juice sac near the column.

关 键 词：琯溪蜜柚 PME 克隆 实时荧光定量PCR 

分 类 号：S813.3[农业科学—畜牧学]