HO-1对肝癌细胞细胞周期调控因子P16和P53的影响  

Role of Heme Oxygenase-1 in the Liver Cancer Cell Cycle Regulation Factors P16 and P53

作　　者：高涵[1] 周涛[2] 张春晶[1] 李淑艳[1] 王丽萍[1] 刘哲丞[1] 

机构地区：[1]齐齐哈尔医学院生物化学与分子生物学教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院第一附属医院普通外科,黑龙江齐齐哈尔161006

出　　处：《临床医药文献电子杂志》2015年第10期1787-1787,1789,共2页Electronic Journal of Clinical Medical Literature

基　　金：黑龙江省齐齐哈尔市科学技术计划项目(合同编号SFGG-201338)

摘　　要：目的观察HO-1对人肝癌细胞HeP G2细胞周期调控因子的影响。方法构建含有野生型和突变型HO-1基因的重组载体,建立稳定表达的HeP G2肝癌细胞系。在HO-1表达改变的稳转细胞系中,利用半定量RT-PCR、Western印迹检测转染细胞系中P16和P53的表达水平。结果实现了野生型和突变型HO-1在HeP G2细胞中的过表达;野生型和突变型HO-1过表达均能诱导P16和P53的表达。结论 HO-1过表达诱导了抑癌基因P16和P53的表达。ObjectiveTo elucidate the effect of heme oxygenase-1 on cell cycle regulator in HepG2 cell line.MethodsTo construct the eukaryotic expression vectors of wild-type and mutant HO-1. To stable expression HepG2 cell line.In the expression of HepG2 cell line using RT-PCR and Western blot to detect the expression of P16 and P53.ResultsHepG2 cell lines of Wild type and mutant HO-1 overexpression were established successfully. Overexpression of wild type and mutant HO-1 can induce the expression of tumor suppressor P16 and P53. ConclusionHO-1 affect the expression of tumor suppressor gene of P16 and P53.

关 键 词：血红素加氧酶-1 肝癌 细胞周期调控因子 

分 类 号：R730.2[医药卫生—肿瘤]