133例乙型肝炎病毒X基因测序结果分析  

Sequencing analysis of hepatitis B virus X genes from 133 cases of patients

作　　者：高慧 何金花 崔美玲 陈伟明 潘莲娣 

机构地区：[1]广州市番禺区中心医院检验科,广东广州511400

出　　处：《海南医学》2015年第7期1001-1004,共4页Hainan Medical Journal

基　　金：广州市番禺区科技计划项目(编号:2012-Z-03-17)

摘　　要：目的对乙型肝炎病毒(HBV)感染者血清标本的HBV X基因进行测序,以了解其突变情况。方法从广州市番禺区133例HBV感染者的血清标本中提取HBV DNA,以PCR方法扩增HBV X基因,再进行测序,根据患者不同病程将其分为乙肝携带组(ASC组)36例、慢性肝炎组(CHB组)40例、肝硬化组(LC组)34例和肝癌组(HCC组)23例,并对结果进行综合比较分析。结果人均碱基突变数ASC组为(5.17±0.86)、CHB组为(5.05±0.80)、LC组为(3.43±0.59),HCC组为(2.05±0.44),ASC组、CHB组和LC组比较差异均无统计学意义(P>0.05),而HCC组均低于ASC组、CHB组、LC组,差异均有统计学意义(P<0.05);HBV X基因突变检出率排前4位的位点是:A1762T联合G1764A双变异为85例(63.91%),T1719G联合G1721A同时变异为77例(57.89%),T1544A为62例(46.62%),T1753C为45例(33.83%)。前4位突变检出率均在LC组中最高(P<0.05)。结论对HBV X基因的高突变位点进行检测为肝硬化及肝癌的发病机制提供线索,对尽早预防和预测肝硬化及肝癌具有重要意义。Objective To understand the mutation of hepatitis B virus X gene by gene sequencing from HBV infected serum. Methods HBV DNAs were extracted from the serum of 133 infectors from Panyu area, and HBV X genes were amplified by PCR and then sequenced. According to the different courses of disease, patients were divided into four groups：36 cases of hepatitis B virus carrier （ASC）, 40 cases of chronic hepatitis （CHB）, 34 cases of liv-er cirrhosis （LC） and 23 cases of hepatocellular carcinoma （HCC）. And the results were comprehensively and compara-tively analyzed. Results The mutation numbers per capita of four groups were as follows：ASC group （5.17 ＆#177; 0.86）, CHB group （5.05＆#177;0.80）, LC group （3.43＆#177;0.86） and HCC group （2.05＆#177;0.44）. And there were no statistically significant difference between the ASC group, CHB group and LC group （P〉0.05）, while the number in HCC group was lower than that in the other three groups （P〈0.05）. The top 4 sites of HBV X gene mutation detection rates were：85 cases of A1762T/G1764A double mutation （63.91%）, 77 cases of T1719G/G1721A simultaneous mutation （57.89%）, 62 cases of T1544A （46.62%） and 45 cases of T1753C （33.83%）. The top 4 sites of mutation detection rates were all highest in LC group （P〈0.05）. Conclusion Detection of the high mutation site of HBV X gene could help provide clues for study-ing the mechanism of liver cirrhosis and liver cancer, which also presents significant reference for clinical practice.

关 键 词：肝炎病毒 乙型 测序 X基因 基因型 变异 肝硬化 肝癌 

分 类 号：R512.62[医药卫生—内科学]