海南岛海防林替代树种的筛选——基于土壤微生物群落功能多样性方法  

Selection of alternative tree species for coastal protective forest in Hainan Island——Based on the functional diversity of soil microbial community

作　　者：钟圣赟 苗妮娜 李昱言 郝清玉[1] 

机构地区：[1]热带动植物生态学省部共建教育部重点实验室/海南师范大学生命科学学院,海南海口571158

出　　处：《广东农业科学》2015年第5期119-126,共8页Guangdong Agricultural Sciences

基　　金：国家科技支撑计划项目(2012BAC18B04);2013年校级研究生创新项目

摘　　要：运用Biolog-ECO技术,以万宁沿海3个地区8种优势植物群落为研究对象,研究了不同植被类型、不同土层土壤微生物群落碳源代谢的多样性。结果表明:(1)土壤微生物对单一碳源的利用强度随培养时间的增加呈递增趋势,上层土对碳源的总体利用能力较下层土的强;(2)两个土层的Shannon多样性指数榄仁树均最低,上层土白楸最高,下层土蕨类+野牡丹+桃金娘等最高;(3)土壤微生物群落对4类碳源的利用率因植被类型而异,且糖类是导致上层土壤微生物代谢差异的敏感碳源;糖类和氨基酸是导致下层土壤微生物代谢差异的敏感碳源;(4)土壤微生物对单一碳源的利用强度与其多样性呈极显著正相关。Based on Biolog-ECO plate technology, the carbon source metabolism diversity of different vegetation types and soil microbial communities of different soil layers under 8 single species-dominated communities distributing in 3 coastal areas of Wanning were analyzed. The results showed that the utilization intensity of soil microbe to single carbon source increased with the increase of incubation time, and the utilization intensity of topsoil microbe to the carbon source was greater than that of subsoil. Shannon diversity indexes in two soil layers indicated that Terminalia catappa was the lowest, Mallotus paniculatus was the highest in the topsoil, and Dicranopteris ＋Melastoma＋Rhodomyrtus tomentosa was the highest in the subsoil. The utilization ratio of soil microbial community to4 kinds of carbon sources varied according to the different vegetation types, and the sugar was the sensitive carbon source to cause the differences of soil microbial metabolism in topsoil, sugars and amino acids were the sensitive carbon sources to cause the differences of soil microbial metabolism in subsoil. There was a positive correlation between the utilization intensity of soil microbial to a single carbon source and its diversity.

关 键 词：土壤 微生物多样性 AWCD Biolog-ECO 天然海防林 

分 类 号：S154.36[农业科学—土壤学]