水库贫营养异养硝化-好氧反硝化菌Sxf14的脱氮特性  

Nitrogen removal characteristics of the heterotrophic nitrification-aerobic denitrification bacterium Acinetobacter sp. Sxf14

作　　者：黄廷林[1] 何秀秀 张海涵[1] 周石磊[1] 白士远 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《应用与环境生物学报》2015年第2期201-207,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家科技支撑计划课题(2012BAC04B02);国家自然科学基金项目(51208413)资助~~

摘　　要：为利用生物强化法降低微污染源水中的氮素,从水库沉积物中筛选到一株好氧反硝化细菌Sx f14.通过扫描电镜和16S r RNA序列分析,鉴定其为不动杆菌属(Acinetobacter sp.),命名为Acinetobacter sp.Sxf14.同时,对该菌株脱氮特性进行研究,并将其接种到C/N(总有机碳与总氮的比值)为1.2的微污染水库源水中,以探究其对实际源水总氮的去除效果.结果显示:Sxf14能以硝酸盐和亚硝酸盐为唯一氮源进行好氧反硝化.反应48 h后,NO3--N和NO2--N的去除率分别达74.84(±0.86)%和40.52(±1.49)%,TN去除率最高达到65.07(±1.56)%和41.33(±0.98)%;在以NH4Cl为氮源的异养硝化系统内,该菌在48 h内使NH4+-N浓度由3.73(±0.08)mg/L降到1.28(±0.20)mg/L,氨氮去除率达到65.63(±1.39)%.72 h内,微污染水库源水的TN浓度由2.46(±0.02)mg/L降到1.68(±0.01)mg/L,去除率达到31.7(±0.14)%.因此,菌株Acinetobacter sp.Sxf14具有反硝化能力,能承受较低的碳氮比,降低微污染源水中的氮素,本研究可为微污染水体的菌剂修复技术提供科学依据.In the present study, a heterotrophic nitrification-aerobic denitrification bacteria strain was isolated from thesediment of a drinking water reservoir. Based on Scan Electronic Microscope （SEM） and 16S rRNA gene sequence analyses, the strain was identified as Acinetobacter sp. and named Acinetobacter sp. Sxfl4. The strain was inoculated into the micro- polluted source water （C/N as 1.2）, and the total nitrogen （TN） removal rate was examined. The results showed that the strain could perform nitrification and denitrification well in nitrate medium, nitrite medium, and the reservoir water environment. The removal rate of nitrate reached 74.84±0.86% and that of nitrite reached 40.52±1.49% in 48 hours; the total nitrogen removal rates was up to 65.07±1.56% and 41.33± 0.98%. The concentration of NH4＋-N reduced from 3.73±0.08 mg/L to 1.28±0.20 mg/L, with the removal rate up to 65.63±1.39%. In 72 h, the concentration of TN of micro-polluted source water reduced from 2.46±0.02 mg/L to 1.68±0.01 mg/L, with the removal rate up to 31.7±0.14%. The results of this work demonstrated that strain Sxfl4, with its aerobic denitrification ability and tolerance low carbon nitrogen ratio, can decrease the nitrogen concentration of micropolluted source water, therefore, and may be a promising agent in bioremediation for micro-polluted sotiree water.

关 键 词：贫营养 异养硝化 好氧反硝化 16SrRNA 水库 

分 类 号：X172[环境科学与工程—环境科学] X524