假酸浆多糖提取纯化工艺的研究  

Study on extraction and purification technology of Nicandra polysaccharide

作　　者：姜盼[1] 王遂[1] 齐欣欣[1] 

机构地区：[1]哈尔滨师范大学化学化工学院,哈尔滨150025

出　　处：《哈尔滨商业大学学报（自然科学版）》2015年第1期28-32,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

摘　　要：以假酸浆籽为原料,采用水提法、超声波提取法、微波提取法提取假酸浆多糖,经对各提取过程比较分析可知水提法为最优提取方法,提取条件为料液比1∶55、温度80℃、时间3 h,提取率为6.18%.将水提法得到的假酸浆多糖浓缩液通过醇沉、脱蛋白、脱色等工艺进行纯化,确定了最佳的提取纯化工艺.在最佳的条件下,醇沉后多糖的量可达80.5%,蛋白质脱除率可达70.8%,脱色率可达91.0%.Nicandra polysaccharide was extracted from the seed of Nicandra physaloides （ L. ） Gaertn by the method of water extraction, ultrasonic extraction, microwave extraction, respectively. Through comparative analysis on the extraction process, water extraction achieving 6.18% extraction rate was determined to be the optimal method, in which the ratio of material to liquid was 1 ： 55, the extraction temperature was 80 ℃, and the extraction time was 3 h. The treatments of ethanol precipitation, deproteinization and decolorization were carried out for purifying to form the final polysaccharide. After the ethanol precipitation, the content of polysaccharide reached 80.5% , the protein removal rate reached 70.8% and the decolorization rate reached 91.0%.

关 键 词：假酸浆多糖 提取 纯化 

分 类 号：TQ281[化学工程—有机化工]