急性白血病患者WT1、PRAME、ERG、TRX-2基因表达的检测及其与疾病分型、疗效的关系  

Expression of WT1,PRAME,ERG,TRX-2 gene in acute leukemia patients and its correlation with type and curative effect of the diseases

作　　者：王善娟 

机构地区：[1]重庆市开县人民医院血液科,重庆405400

出　　处：《海南医学院学报》2015年第6期785-787,790,共4页Journal of Hainan Medical University

基　　金：重庆市卫生局医学科研基金资助项目(2012-2-413)~~

摘　　要：目的:研究急性白血病患者肾母细胞瘤基因(WT1)、黑色素瘤优先表达抗原(PRAME)、ETS相关基因(ERG)、硫氧还蛋白-2(TRX-2)基因表达及其与疾病分型、疗效的关系。方法:将本院收治的90例白血病患者纳入研究的白血病组,将同期体检健康者纳入健康组;进一步根据分型将白血病患者分为ALL组和AML组,根据化疗后疗效将白血病患者分为完全缓解组和复发组。采集患者的外周血,检测WT1、PRAME、ERG、TRX-2基因的mRNA含量。结果:白血病组血液中WT1、PRAME、ERG、TRX-2基因的mRNA含量高于对照组,差异有统计学意义(P<0.05);AML组血液中WT1、PRAME、ERG、TRX-2基因的mRNA含量高于ALL组,差异有统计学意义(P<0.05);复发组的血液中WT1、PRAME、ERG、TRX-2基因的mRNA含量高于完全缓解组,差异有统计学意义(P<0.05)。结论:WT1、PRAME、ERG、TRX-2基因异常高表达与白细胞的发生有关,也与疾病的分型、化疗的效果密切相关。Objective： To study the expression of WT1, PRAME, ERG, TRX 2 gene of acute leukemia patients and its correlation with type and curative effect of the diseases. Methods： 90 cases of acute leukemia patients were enrolled as leukemia group, healthy subjects received physical examination in our hospital during the same period were enrolled in healthy group. A- cute leukemia patients were subdivided into ALL group and AML group according to type of diseases; they were also divided into complete remission group and recurrent group according to curative effect. Peripheral blood was collected and mRNA con- tent of WT1, PRAME, ERG, TRX-2 were analyzed. Results： mRNA contents of WT1, PRAME, ERG, TRX-2 in peripheral blood of leukemia group were higher than those of healthy group; mRNA contents of WT1, PRAME, ERG, TRX-2 in periph- eral blood of AML group were higher than those of ALL group; mRNA contents of WT1, PRAME, ERG, TRX-2 in peripher- al blood of recurrent group were higher than those of complete remission group. Conclusion： Abnormally high expression ofWT1, PRAME, ERG, TRX-2 is correlated with occurrence of leukocyte, and also closely correlated with type of diseases and curative effect.

关 键 词：急性白血病 肾母细胞瘤基因(WT1) 黑色素瘤优先表达抗原(PRAME) ETS相关基因(ERG) 硫氧还蛋白-2(TRX-2) 

分 类 号：R733.71[医药卫生—肿瘤]