高原牦牛 黄牛心肌及骨骼肌线粒体抗氧化作用的比较研究  

Research of Antioxidation of Myocardial and Skeletal Muscle Mitochondria in Plateau Yak and Cattle

作　　者：常建军[1] 吴廉甫 李莉[1] 张勤文[1] 林伟山[1] 王勇[1] 

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]黑龙江天翼生物科技有限公司,黑龙江哈尔滨150038

出　　处：《中国兽医杂志》2015年第3期6-8,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目资助(31160489);青海省科技厅项目资助(2013-7-012)

摘　　要：采用酶联免疫分析法(ELISA)和比色法对高原牦牛和黄牛心肌、骨骼肌线粒体琥珀酸脱氢酶(SDH)、细胞色素氧化酶(CCO)、三磷酸腺苷(ATP)、氧化物歧化酶(T-SOD)及丙二醛(MDA)指标进行测定。结果显示,高原牦牛与黄牛心肌、骨骼肌线粒体ATP、SDH、CCO、T-SOD酶活性及MDA含量差异均不显著(P>0.05);高原牦牛与黄牛心肌、骨骼肌胞浆SDH、CCO活性均高于线粒体(P<0.05)。The activities of cardiac and skeletal muscle mitochondria characteristic enzyme Succinic dchydrogenase, Cytochrome oxidas, adenosinetriphosphate, total superoxide dismutase, Maleic Dialdehyde in Plateau Yak and Cattle were determined by using enzyme immunoassay （ELISA） and colorimetric method. The result showed ： there was no significant difference between the activities of SDH, CCO, ATP,T-SOD, MDA in myocardial and skeletal muscle mitochondrion in Plateau Yak and Cattle（P〉0.05） ,The activities of SDH, CCO in myocardial and skeletal muscle cytoplasm in Plateau Yak and Cattle were higher than those of the mitochondria（P〈0.05）.

关 键 词：牦牛 黄牛 线粒体 SDH CCO ATP T-SOD MDA 

分 类 号：S823[农业科学—畜牧学]