甘肃河西地区西门塔尔杂交类群NGB基因第4外显子的遗传变异研究  

Genetic Variation Study of NGB Gene Exon 4 of Simmental Cattle Hybrid Taxon in Gansu Hexi Regions

作　　者：梁春花[1,2] 刘霞[3,2] 罗玉柱[2] 孙雪婧[1,2] 高雪晋 杨阳[1] 曾国敏[3] 杜晓华[1,2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]甘肃省草食动物生物技术重点实验室,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《华北农学报》2015年第2期22-27,共6页Acta Agriculturae Boreali-Sinica

基　　金：甘肃省高等学校基本科研业务费资助项目(2013);甘肃省教育厅研究生导师项目(1102-04)

摘　　要：为研究甘肃河西的临泽、甘州、凉州、金昌、高台5个地区西门塔尔杂交类群NGB基因第4外显子的遗传多态性及其变异特征,采用PCR-SSCP方法检测了283头西门塔尔杂交类群NGB基因第4外显子和部分内含子的多态性,并对群体内各等位基因进行了测序。结果显示:5个地区西门塔尔杂交类群共检测出5个等位基因(A、B、C、D、E),表现为6种基因型(AA、BB、AB、AC、AD、AE)。5个地区西门塔尔杂交类群均能检测到AA、BB、AB 3种基因型,且甘州、凉州、高台西门塔尔杂交类群还分别检测到AC、AD、AE 3种基因型。A等位基因频率在5个群体中最高,为优势等位基因。对不同SSCP带型的对应片段进行测序分析,共发现5个核苷酸突变位点(31 bp C→T、68 bp G→C和G→A、129 bp A→G、207 bp C→T),5处突变中仅第207 bp处单核苷酸突变落在外显子区域,其余均落在内含子区域,但均未导致氨基酸发生改变。χ2检验结果显示,5个地区西门塔尔杂交类群在第207 bp处单核苷酸突变位点上仅凉州、金昌西门塔尔杂交类群处于Hardy-Weinberg平衡状态(P>0.05)。群体遗传学分析结果表明,临泽、甘州、凉州、金昌、高台西门塔尔杂交类群的多态信息含量(PIC)分别为0.358 2,0.383 9,0.427 9,0.368 2,0.394 7,均属于中度多态(0.25<PIC<0.5)。The study was designed to analyze the genetic polymorphism and variability of NGB gene exon 4 of Simmental cattle hybrid taxon in Gansu Linze,Ganzhou,Liangzhou,Jinchang,and Gaotai five regions. DNA fragment containing the exon 4 and part of intron of NGB gene were amplified and genotyped using PCR single-strand confor-mational polymorphism（ SSCP）method. Representative alleles were sequenced for verification of their variations in DNA sequences. The results displayed that there were 5 alleles（ A,B,C,D and E）detected,combined six geno-types as AA,BB,AB,AC,AD and AE. Among them,AA,BB and AB genotypes were all detected in the five re-gions. AC,AD and AE genotypes was detected in Ganzhou,Liangzhou and Gaotai respectively. Allele A was the pre-dominant genotype in five regions. Sequence of the SSCP showed 5 mutations in the DNA fragment（31 bp C→T,68 bp G→C and G→A,129 bp A→G,207 bp C→T）. In which,C→T at 207 bp was located in exon,while the other mutations were located in intron. But all had not leaded to amino acid changed. Chi-square testing indicated that in the 207 bp single nucleotide mutation sites,only Liangzhou,Jinchang Simmental cattle hybrid taxon among the five＆amp;nbsp;regions were in Hardy-Weinberg equilibrium（P〉0. 05）. Population genetics analysis showed the polymorphism in-formation content were 0. 358 2,0. 383 9,0. 427 9,0. 368 2 and 0. 394 7 in Linze,Ganzhou,Liangzhou,Jinchang, Gaotai,respectively,which were all at a moderate level of polymorphism（0. 25 〈PIC〈0. 5）.

关 键 词：西门塔尔杂交类群 NGB基因 外显子 遗传变异 

分 类 号：Q78[生物学—分子生物学]