Functional Marker Development and Effect Analysis of Grain Size Gene GW2 in Extreme Grain Size Germplasm in Rice  

作　　者：ZHANG Ya-dong ZHENG Jia LIANG Yan-li ZHAO Chun-fang CHEN Tao ZHAO Qing-yong ZHU Zhen ZHOU Li-hui YAO Shu ZHAO Ling YU Xing WANG Cai-lin 

机构地区：[1]Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Jiangsu High Quality Rice R & D Center,Nanjing Branch of China National Center for Rice Improvement

出　　处：《Rice science》2015年第2期65-70,共6页水稻科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No.31271678);the Jiangsu Agricultural Scientific Self-Innovation Fund (Grant No.CX[12]1003);Jiangsu Province Agricultural Science and Technology Support Program (Grant No.BE2013301)

摘　　要：GW2 is an important gene that regulates grain width and weight. We used cDNA clone to obtain the sequences of GW2 from large- and small-grained rice varieties, TD70 and Kasalath, respectively. Then, we developed a dCAPS （derived cleaved amplified polymorphic sequence） marker on the basis of the sequence difference between functional and nonfunctional GW2 genes to analyze the genotypes and phenotypes of recombinant inbred lines. Results showed that the sequence of GW2To7~ had a single nucleotide deletion at site 316 that generates a termination codon. This codon terminated the GW2 protein in advance. By contrast, the sequence of GW2Kasalath encoded an intact protein. A novel dCAPS marker was designed in accordance with a base A deletion at site 316 of the sequence. After the PCR product was digested by Apol, TD70 showed 21 and 30 bp fragments, and Kasalath showed a 51 bp fragment. Up to 82 lines contained GW2TDTO, and 158 lines contained GW2Kasalath. The lines that contained TD70 alleles displayed substantial increases in width and 1000-grain weight. This result suggested that GW2 played a critical role in rice breeding.GW2 is an important gene that regulates grain width and weight. We used cDNA clone to obtain the sequences of GW2 from large- and small-grained rice varieties, TD70 and Kasalath, respectively. Then, we developed a dCAPS （derived cleaved amplified polymorphic sequence） marker on the basis of the sequence difference between functional and nonfunctional GW2 genes to analyze the genotypes and phenotypes of recombinant inbred lines. Results showed that the sequence of GW2To7~ had a single nucleotide deletion at site 316 that generates a termination codon. This codon terminated the GW2 protein in advance. By contrast, the sequence of GW2Kasalath encoded an intact protein. A novel dCAPS marker was designed in accordance with a base A deletion at site 316 of the sequence. After the PCR product was digested by Apol, TD70 showed 21 and 30 bp fragments, and Kasalath showed a 51 bp fragment. Up to 82 lines contained GW2TDTO, and 158 lines contained GW2Kasalath. The lines that contained TD70 alleles displayed substantial increases in width and 1000-grain weight. This result suggested that GW2 played a critical role in rice breeding.

关 键 词：RICE grain size GW2 gene derived cleaved amplified polymorphic sequence PHENOTYPE 

分 类 号：S511[农业科学—作物学] TP391[自动化与计算机技术—计算机应用技术]