石蒜碱对人胃癌细胞体外增殖的影响  

The Influence of Lycorisradiata Alkali on the Proliferation of Human Gastric Cancer Cells Cultured in Vitro

作　　者：刘希宇 贺小英[1] 王凤梅[2] 程腾[1] 马利兵[1] 

机构地区：[1]内蒙古科技大学数理与生物工程学院,内蒙古包头014010 [2]包头轻工职业技术学院,内蒙古包头014035

出　　处：《信阳师范学院学报（自然科学版）》2015年第2期173-176,共4页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31160245);内蒙古自治区自然科学基金项目(2012MS0503);内蒙古自治区高等学校青年科技英才支持计划项目

摘　　要：以体外培养的h GCCs MGC-803细胞系为研究对象,采用不同浓度石蒜碱(5、2.5、1.25μmol/L)进行处理,倒置显微镜下观察其生长状态,同时采用血球计数板统计其体外增殖率,采用流式细胞技术检测处于不同细胞周期的细胞比例.结果表明,石蒜碱处理对h GCCs的体外增殖具有一定的抑制作用,其中5μmol/L石蒜碱对h GCCs的抑制作用最为明显.显微观察和计数分析结果表明,5μmol/L石蒜碱处理的h GCCs活力最低,细胞凋亡现象最明显.细胞周期检测的结果显示石蒜碱处理会使h GCCs增殖趋缓,细胞周期延长.The h GCCs were treated with different concentration of lycorisradiata alkali（ 5、2. 5、1. 25 μmol / L respectively）,growth status were observed by the inverted microscope,and proliferation rate were count by hemocytometer,the proportion of h GCCs in different cell cycles were detected by flow cytometry. The results showed that the proliferation of h GCCs was suppressed by treated with lycorisradiata alkali,the most obvious suppressed of h GCCs was 5μmol /L treatment group. Through microscopic observation and counting analysis results,it has been found that the vitality of h GCCs was the lowest and the cells apoptosis was the most obvious by treated of 5 μmol / L lycorisradiata alkali. The result of cell cycle detection revealed that by treated with lycorisradiata alkali,the reproduction of h GCCs became slow and cell cycle became extended.

关 键 词：人胃癌细胞 细胞增殖 石蒜碱 细胞活力 细胞周期 

分 类 号：Q256[生物学—细胞生物学]